Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role in canalicular bile secretion

The Journal of Biological Chemistry
Robert C HuebertNicholas F LaRusso

Abstract

Although bile formation requires that large volumes of water be rapidly transported across liver epithelia, including hepatocytes, the molecular mechanisms by which water is secreted into bile are obscure. The aquaporins are a family of 10 channel-forming, integral membrane proteins of approximately 28 kDa numbered 0-9 that allow water to rapidly traverse epithelial barriers in several organs including kidney, eye, and brain. We found transcripts of three of 10 aquaporins in hepatocytes (aquaporin 8 aquaporin 9 > aquaporin 0) by reverse transcription-polymerase chain reaction and quantitative ribonuclease protection assays; immunohistochemistry confirmed the presence of these three proteins in liver. Immunoblots of subcellular fractions of hepatocytes showed enrichment of aquaporins 0 and 8 in microsomes and canalicular plasma membranes; aquaporin 9 was enriched only in basolateral plasma membranes. Immunofluorescence of hepatocyte couplets confirmed the intracellular/canalicular localization of aquaporins 0 and 8 and the basolateral localization of aquaporin 9. Upon exposure of couplets to a choleretic stimulus (i.e. dibutyryl cAMP), aquaporin 8 redistributed to the canalicular plasma membrane; the subcellular distributions of...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
May 1, 1990·Journal of Hepatology·J Graf, J L Boyer
Nov 1, 1988·Seminars in Liver Disease·P J Meier
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J GrafJ L Boyer
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·S K RobertsN F LaRusso
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·A BenedettiJ L Boyer
Mar 22, 1996·The Journal of Biological Chemistry·M YanoN F LaRusso
Oct 11, 1996·The Journal of Biological Chemistry·M EchevarríaG Frindt
Jun 5, 1997·Nature·T WalzA Engel
Aug 28, 1997·Biochemical and Biophysical Research Communications·K IshibashiS Sasaki
Dec 31, 1997·The Journal of Biological Chemistry·Y KoyamaI Kihara
May 8, 1999·Journal of the American Society of Nephrology : JASN·A S Verkman
Nov 5, 1999·Biochemical and Biophysical Research Communications·Y IshikawaH Ishida
Feb 1, 2000·Molecular Medicine Today·L S KingP Agre
May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A MarinelliN F LaRusso
Jun 29, 2000·Annual Review of Biochemistry·M BorgniaP Agre
Jul 13, 2000·Journal of Molecular Biology·D FotiadisA Engel
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·K N LazaridisN F LaRusso
Oct 12, 2000·Biochemical and Biophysical Research Communications·M ElkjaerS Nielsen
Feb 15, 2001·American Journal of Physiology. Cell Physiology·A G GianoukakisT J Smith
Jul 5, 2001·Archives of Histology and Cytology·T TaniT Yamamoto
Aug 30, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E CovaN F LaRusso
Feb 8, 2002·Gastroenterology·Anatoly I MasyukNicholas F LaRusso

❮ Previous
Next ❯

Citations

Jul 1, 2005·Pflügers Archiv : European journal of physiology·Lars M HolmThomas Zeuthen
Oct 22, 2003·Journal of Hepatology·Piero PortincasaGiuseppe Calamita
Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Domenico FerriGiuseppe Calamita
Feb 14, 2007·Journal of Gastroenterology and Hepatology·Satoru KawaiYuji Nimura
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Dec 26, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anja WellejusThomas E Jonassen
Dec 6, 2008·World Journal of Gastroenterology : WJG·Marcelo G RomaAldo D Mottino
Dec 17, 2008·World Journal of Gastroenterology : WJG·Guillermo-L LehmannRaul-A Marinelli
Nov 22, 2013·World Journal of Gastroenterology : WJG·Vasiliy Ivanovich Reshetnyak
Sep 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jay D HortonJoseph L Goldstein
May 13, 2014·Molecular Genetics and Genomics : MGG·Wei Liu, Hua Ye
Feb 18, 2016·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Apr 11, 2012·Molecular Aspects of Medicine·Piero Portincasa, Giuseppe Calamita
Jul 11, 2009·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Srikanth PadmaIain H McKillop
Sep 28, 2007·Neoplasia : an International Journal for Oncology Research·Yasuhiko HayashiMarsha J Merrill
Feb 6, 2010·Biochemical and Biophysical Research Communications·Leandro R SoriaGiuseppe Calamita
Sep 21, 2007·Neuroscience Letters·Ianors IandievAndreas Bringmann
Jan 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Amelia MazzoneNicholas F LaRusso
Jul 30, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Guillermo L Lehmann, Raúl A Marinelli
Mar 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kulandaiappan VaradarajRichard T Mathias
Jan 5, 2008·European Journal of Clinical Investigation·P PortincasaG Calamita
Jan 10, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Leandro R SoriaRaúl A Marinelli
Oct 21, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Ashley M LaknerLaura W Schrum
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Robert C HuebertVijay H Shah
Mar 15, 2005·Journal of Hepatology·Raúl A MarinelliNicholas F Larusso
Nov 30, 2004·Trends in Molecular Medicine·Raúl A Marinelli
Nov 2, 2005·Human Pathology·Peter R MazalAndrea Haitel
Sep 13, 2005·Metabolism: Clinical and Experimental·Dae-Ho LeeMin-Young Chung
Aug 8, 2015·Cryobiology·Balasubramanian K KumarCharles Y Lee
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Flavia I CarrerasRaúl A Marinelli
May 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sergio A GradiloneRaúl A Marinelli
Feb 1, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anatoliy I Masyuk, Nicholas F LaRusso
Jul 2, 2015·Histochemistry and Cell Biology·Françoise GregoireChristine Delporte
Sep 3, 2008·Drug Metabolism and Pharmacokinetics·Yuriko OhgusuHiroaki Yuasa
Sep 18, 2014·Biochemical and Biophysical Research Communications·S Sindhu Kumari, Kulandaiappan Varadaraj
Mar 7, 2006·Biochimica Et Biophysica Acta·Katalin KalmanJames E Hall
Mar 22, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Julieta MarroneRaúl A Marinelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.