Expression and localization of aromatase during fetal mouse testis development

Basic and Clinical Andrology
Caroline BordayRené Habert

Abstract

Both androgens and estrogens are necessary to ensure proper testis development and function. Studies on endocrine disruptors have highlighted the importance of maintaining the balance between androgens and estrogens during fetal development, when testis is highly sensitive to environmental disturbances. This balance is regulated mainly through an enzymatic cascade that converts irreversibly androgens into estrogens. The most important and regulated component of this cascade is its terminal enzyme: the cytochrome p450 19A1 (aromatase hereafter). This study was conducted to improve our knowledge about its expression during mouse testis development. By RT-PCR and western blotting, we show that full-length aromatase is expressed as early as 12.5 day post-coitum (dpc) with maximal expression at 17.5 dpc. Two additional truncated transcripts were also detected by RT-PCR. Immunostaining of fetal testis sections and of gonocyte-enriched cell cultures revealed that aromatase is strongly expressed in fetal Leydig cells and at variable levels in gonocytes. Conversely, it was not detected in Sertoli cells. This study shows for the first time that i) aromatase is expressed from the early stages of fetal testis development, ii) it is express...Continue Reading

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Citations

Feb 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·René HabertGabriel Livera
Nov 20, 2016·Reproduction in Domestic Animals = Zuchthygiene·B C Braun, K Jewgenow
Jan 15, 2021·Differentiation; Research in Biological Diversity·Rex A HessBarry T Hinton

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Methods Mentioned

BETA
PCR

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