Expression and Localization of CaBP Ca2+ Binding Proteins in the Mouse Cochlea

PloS One
Tian YangAmy Lee

Abstract

CaBPs are a family of EF-hand Ca2+ binding proteins that are structurally similar to calmodulin. CaBPs can interact with, and yet differentially modulate, effectors that are regulated by calmodulin, such as Cav1 voltage-gated Ca2+ channels. Immunolabeling studies suggest that multiple CaBP family members (CaBP1, 2, 4, and 5) are expressed in the cochlea. To gain insights into the respective auditory functions of these CaBPs, we characterized the expression and cellular localization of CaBPs in the mouse cochlea. By quantitative reverse transcription PCR, we show that CaBP1 and CaBP2 are the major CaBPs expressed in mouse cochlea both before and after hearing onset. Of the three alternatively spliced variants of CaBP1 (caldendrin, CaBP1-L, and CaBP1-S) and CaBP2 (CaBP2-alt, CaBP2-L, CaBP2-S), caldendrin and CaBP2-alt are the most abundant. By in situ hybridization, probes recognizing caldendrin strongly label the spiral ganglion, while probes designed to recognize all three isoforms of CaBP1 weakly label both the inner and outer hair cells as well as the spiral ganglion. Within the spiral ganglion, caldendrin/CaBP1 labeling is associated with cells resembling satellite glial cells. CaBP2-alt is strongly expressed in inner hair c...Continue Reading

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Citations

Feb 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Maria Magdalena PicherTobias Moser
Aug 2, 2018·Physiological Reviews·Tina PangrsicAlexandra Koschak
Oct 12, 2018·Nature·Teerawat WiwatpanitJaime García-Añoveros
Mar 6, 2019·Cold Spring Harbor Perspectives in Biology·Robert D BurgoyneLee P Haynes
May 20, 2020·Gene Therapy·Yiyang LanGuisheng Zhong
Dec 19, 2019·Pflügers Archiv : European journal of physiology·Nadine J OrtnerJörg Striessnig
Apr 18, 2018·Hearing Research·Tian YangAmy Lee
Sep 8, 2021·Frontiers in Molecular Neuroscience·David OestreicherTina Pangrsic

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Methods Mentioned

BETA
reverse transcription PCR
PCR

Software Mentioned

StepOne
CellSens Standard
Adobe Photoshop
GraphPad Prism6
FluoView

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