Expression and localization of cytochrome P450 17 alpha-hydroxylase/c17,20-lyase in the avian brain

Brain Research
Masahiro MatsunagaK Tsutsui

Abstract

Steroids synthesized de novo from cholesterol in the brain are generally called neurosteroids. We have recently demonstrated, using biochemical and molecular biological methods, that certain structures in the quail brain possess cytochrome P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase/Delta(5)-Delta(4)-isomerase (3beta-HSD) and produce pregnenolone, pregnenolone sulfate and progesterone. To clarify the biosynthetic pathway of neurosteroids in the avian brain, therefore, we examined the expression of messenger RNA (mRNA) encoding for the enzyme cytochrome P450 17alpha-hydroxylase/c17,20-lyase (P450(17alpha,lyase)), which converts pregnenolone to dehydroepiandrosterone via 17alpha-hydroxypregnenolone or progesterone to androstenedione via 17alpha-hydroxyprogesterone. RT-PCR analysis followed by Southern hybridization indicated the expression of P450(17alpha,lyase) mRNA in the brain of sexually mature birds without a clear-cut sex difference. Employing biochemical techniques combined with HPLC analysis, the conversion of progesterone to 17alpha-hydroxyprogesterone was also found in brain slices of the mature male. P450(17alpha,lyase) mRNA was detected in various brain regions, but there was a cle...Continue Reading

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Citations

Mar 1, 2008·Endocrinology·Kazuyoshi Tsutsui
Feb 10, 2009·Journal of Neurochemistry·Elizabeth SujkovicJonathan P Fry
May 22, 2009·Annals of the New York Academy of Sciences·Shogo HaraguchiKazuyoshi Tsutsui
May 17, 2014·Frontiers in Cellular Neuroscience·Kazuyoshi Tsutsui, Shogo Haraguchi
Sep 10, 2003·Endocrinology·Hirotaka SakamotoKazuyoshi Tsutsui
Oct 24, 2007·Journal of Neurochemistry·Devaleena S PradhanKiran K Soma
Oct 4, 2008·Endocrinology·Yimin ShiSynthia H Mellon
Mar 22, 2012·Endocrinology·Marcus D SchonemannSynthia H Mellon
Jul 4, 2018·Frontiers in Endocrinology·John C WingfieldKazuyoshi Tsutsui
Aug 12, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Kazuyoshi TsutsuiKazuyoshi Ukena
May 22, 2009·Annals of the New York Academy of Sciences·Jean-Luc Do RegoHubert Vaudry
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masahiro MatsunagaKazuyoshi Tsutsui
Jan 1, 2013·Journal of Experimental Neuroscience·Kazuyoshi TsutsuiYoshitaka Fukada
Dec 11, 2002·European Journal of Biochemistry·Kaori SawadaKazuyoshi Tsutsui
Mar 13, 2020·FASEB BioAdvances·Kazuyoshi Tsutsui, Shogo Haraguchi
Nov 27, 2015·General and Comparative Endocrinology·Yuki OguraKazuyoshi Tsutsui
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Kazuyoshi TsutsuiKazuyoshi Ukena
Jun 16, 2004·Neuroscience Research·Kazuyoshi TsutsuiKazuyoshi Ukena
Nov 23, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Kazuyoshi Tsutsui
Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry

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