Expression and localization of growth hormone-releasing hormone messenger ribonucleic acid in rat placenta: in vitro secretion and regulation of its peptide product

Endocrinology
A N MargiorisG P Chrousos

Abstract

The placenta is the source of many hypothalamic peptides. We now report that GH-releasing hormone (GHRH) mRNA was detectable in rat placenta. On Northern blot hybridization analysis, the size of the placental GHRH transcript was the same with the putative GHRH precursor mRNA. On in situ hybridization, the cells expressing the GHRH mRNA had the morphological characteristics of cytotrophoblasts. In addition, immunoreactive (IR) postranslational products of GHRH were present in rat placental extracts and in the effluent of in vitro perifused placentae. On gel filtration chromatography, the bulk of IR-GHRH present in placental extracts and perifusion effluent had the same size as the authentic hypothalamic GHRH (5.2K). A higher mol wt form of IR-GHRH of about 10K was also present and may represent the pro-GHRH predicted from the sequence of the GHRH cDNA. The mean basal release of IR-GHRH in the perifusion effluent from full thickness rat placental fragments was 337.3 +/- 38.5 (+/- SE; n = 48) pg/10 min fraction.g tissue. Depolarization by 56 mM KCl increased the concentration of the secreted immunoreactive peptide to 632.2 +/- 50.5. A 10-min exposure to 8-bromo-cAMP caused an immediate, monophasic, and dose-dependent increase in I...Continue Reading

Citations

Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nikolina DioufaHippokratis Kiaris
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S González-Crespo, A Boronat
Jan 1, 1994·Molecular Aspects of Medicine·G Desoye, E Shafrir
Sep 19, 2009·Pathology, Research and Practice·Maria SatraNicholas C Vamvakopoulos
Feb 25, 2005·Vitamins and Hormones·Hippokratis KiarisAnastasios Kalofoutis
Mar 5, 2003·Pharmacological Reviews·Kelly E MayoDaniel J Drucker
Mar 3, 1998·Molecular Endocrinology·S PetersennH M Schulte
Apr 30, 1999·Physiological Reviews·E E MüllerD Cocchi
Aug 16, 2006·DNA and Cell Biology·Marta L FiorottoRuxandra Draghia-Akli

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