PMID: 11900964Mar 20, 2002Paper

Expression and localization of IGF family members in bovine antral follicles during final growth and in luteal tissue during different stages of estrous cycle and pregnancy

Domestic Animal Endocrinology
D SchamsW M Amselgruber

Abstract

The objectives of the study were to monitor the detailed pattern for mRNA expression (RT-PCR and RPA) of IGFs, IGFR-1, IGFBPs, GHR and localization of protein (immunohistochemistry) for IGF-1 and IGFR-1 in bovine follicle classes during final maturation and different corpus luteum (CL) stages during estrous cycle and during pregnancy. A relative high expression of IGF-1 in theca interna (TI) was observed before selection (E<0.5ng/mL). In GC, mRNA expression increased after selection. In contrast, IGF-2 was mainly expressed in the TI. The IGFR-1 mRNA was present in the TI and GC with increasing levels during final development. The expression results were confirmed by localization of IGF-1 and IGFR-1 proteins in GC and TI. There is clear evidence for the local expression of IGFBPs in TI and GC compartment with clear regulatory differences. In CL, the highest mRNA expression of IGF-1, IGF-2 and IGFR-1 was observed during early luteal phase, followed by a decrease, and then by a tendency of an increase during the mid and late luteal phases of the cyclic CL. This level remained low during pregnancy. Intense immunostaining for IGFR-1 in CL was observed mainly in large luteal cells. Evidence for a mRNA for all six IGFBPs were obtained...Continue Reading

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Citations

Nov 26, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M A VelazquezH Niemann
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·M A Beg, O J Ginther
Apr 30, 2013·Research in Veterinary Science·M AlbrizioR L Sciorsci
Jul 1, 2004·Reproduction in Domestic Animals = Zuchthygiene·D Schams, B Berisha
Mar 21, 2007·Anatomia, histologia, embryologia·H H OrtegaE J Gimeno
Feb 22, 2008·Molecular Reproduction and Development·Michael K SkinnerEric E Nilsson
Jan 5, 2011·Reproduction in Domestic Animals = Zuchthygiene·M SarkarB Berisha
Nov 13, 2009·Molecular Reproduction and Development·M SarkarBajram Berisha
May 25, 2012·Reproduction in Domestic Animals = Zuchthygiene·H KliemD Schams
Oct 27, 2004·Molecular and Cellular Endocrinology·L J SpicerD T Allen
Jul 7, 2005·Domestic Animal Endocrinology·B Berisha, D Schams
Apr 1, 2014·Journal of Dairy Science·S P M AungierM A Crowe
Nov 3, 2007·Reproduction in Domestic Animals = Zuchthygiene·M P GreenM G Hunter
Aug 16, 2008·Domestic Animal Endocrinology·M A VelazquezD C Wathes
Aug 8, 2018·Endocrinology·Ketan Shrestha, Rina Meidan
Sep 8, 2020·Veterinary Medicine and Science·Diogo J CardilliJosé F Pérez-Gutiérrez
Apr 22, 2016·The Journal of Reproduction and Development·Takashi Shimizu
Nov 30, 2019·Frontiers in Endocrinology·Emina IpsaKevin N Keane
Apr 16, 2020·Science China. Life Sciences·Tahir MuhammadZi-Jiang Chen
Aug 6, 2019·Frontiers in Endocrinology·Jesús Devesa, Diego Caicedo

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