Expression and localization of myosin VI in developing mouse spermatids

Histochemistry and Cell Biology
Przemysław ZakrzewskiMarta Lenartowska

Abstract

Myosin VI (MVI) is a versatile actin-based motor protein that has been implicated in a variety of different cellular processes, including endo- and exocytic vesicle trafficking, Golgi morphology, and actin structure stabilization. A role for MVI in crucial actin-based processes involved in sperm maturation was demonstrated in Drosophila. Because of the prominence and importance of actin structures in mammalian spermiogenesis, we investigated whether MVI was associated with actin-mediated maturation events in mammals. Both immunofluorescence and ultrastructural analyses using immunogold labeling showed that MVI was strongly linked with key structures involved in sperm development and maturation. During the early stage of spermiogenesis, MVI is associated with the Golgi and with coated and uncoated vesicles, which fuse to form the acrosome. Later, as the acrosome spreads to form a cap covering the sperm nucleus, MVI is localized to the acroplaxome, an actin-rich structure that anchors the acrosome to the nucleus. Finally, during the elongation/maturation phase, MVI is associated with the actin-rich structures involved in nuclear shaping: the acroplaxome, manchette, and Sertoli cell actin hoops. Since this is the first report of M...Continue Reading

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Citations

Mar 13, 2020·ELife·J Aaron CrapsterJames K Chen
May 16, 2020·Biology of Reproduction·Przemysław ZakrzewskiMarta Lenartowska
Nov 25, 2019·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Apr 23, 2020·Human Reproduction Update·Christiane PleugerMoira K O'Bryan
Jan 3, 2021·Histochemistry and Cell Biology·Przemysław ZakrzewskiFolma Buss
Jun 17, 2021·Biology of Reproduction·Wenfeng XiongZhugang Wang

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Methods Mentioned

BETA
PCR
Protein Assay
electrophoresis
transmission electron microscopy
GTPases

Software Mentioned

cellB

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