Expression and localization of rat aldo-keto reductases and induction of the 1B13 and 1D2 isoforms by phenolic antioxidants

Drug Metabolism and Disposition : the Biological Fate of Chemicals
A Kenneth MacLeodJohn D Hayes

Abstract

The aldo-keto reductase (AKR) phase I drug metabolism enzyme superfamily is implicated in detoxification or bioactivation of a wide variety of carbonyl-bearing compounds. In this study, we have used antibodies raised against purified recombinant rat AKR isoforms 1A3, 1B4, 1C9, 1D2, and 7A1 to characterize the expression profile of these superfamily members in the rat and define their localization by immunohistochemistry. Western blotting showed that AKR1A3, AKR1B4, and AKR1C9 are ubiquitously expressed, whereas AKR1D2 and AKR7A1 are present in liver, adrenal gland, and kidney, with the latter also present in testis, spleen, and stomach. Immunohistochemical analysis of the kidney demonstrated the localization of AKR1A3 in proximal convoluted tubules, AKR1B4 in the loop of Henle, and AKR1C9 in the pars recta S3 segment of proximal tubules. We also report localization of AKR1B4 in the adrenal gland (parenchymal cells of the zona reticularis) and testis (Sertoli cells and late spermatids), of AKR1D2 in the liver (hepatocyte nuclei), and of AKR7A1 in the pancreatic duct and bronchiolar epithelium. Previous studies have shown that expression of AKR7A1 is induced in response to dietary administration of the phenolic antioxidants butyl...Continue Reading

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Citations

Feb 12, 2014·Steroids·Mo Chen, Trevor M Penning
Mar 18, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoshiko Konishi, Kei-Ichi Sugiyama
Jun 21, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Munzir M E AhmedJames Y Yang
Jan 27, 2021·International Journal of Molecular Sciences·Naoko NiimiKazunori Sango

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