Expression and localization of phosphoenolpyruvate carboxylase in developing and germinating wheat grains

Plant Physiology
M C GonzalezFrancisco Javier Cejudo

Abstract

Phosphoenolpyruvate carboxylase (PEPC) activity and corresponding mRNA levels were investigated in developing and germinating wheat (Triticum aestivum) grains. During grain development PEPC activity increased to reach a maximum 15 d postanthesis. Western-blot experiments detected two main PEPC polypeptides with apparent molecular masses of 108 and 103 kD. The most abundant 103-kD PEPC subunit remained almost constant throughout the process of grain development and in the scutellum and aleurone layer of germinating grains. The less-abundant 108-kD polypeptide progressively disappeared during the second half of grain development and was newly synthesized in the scutellum and aleurone layer of germinating grains. PEPC mRNA was detected throughout the process of grain development; however, in germinating grains PEPC mRNA accumulated transiently in the scutellum and aleurone layer, showing a sharp maximum 24 h after imbibition. Immunolocalization studies revealed the presence of the enzyme in tissues with a high metabolic activity, as well as in the vascular tissue of the crease area of developing grains. A clear increase in PEPC was observed in the scutellar epithelium of grains 24 h after imbibition. The data suggest that the tran...Continue Reading

References

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Citations

Mar 31, 2004·Plant Physiology and Biochemistry : PPB·Georgios AivalakisJ B Drossopoulos
Jun 5, 2003·Archives of Biochemistry and Biophysics·Mitsue Miyao, Hiroshi Fukayama
Aug 30, 2008·The Journal of Biological Chemistry·R Glen UhrigWilliam C Plaxton
Dec 3, 2010·BMC Plant Biology·Silvia DonniniGraziano Zocchi
Nov 30, 2013·Journal of Experimental Botany·Isabel Ruiz-BallestaCristina Echevarría
Sep 13, 2008·The Plant Journal : for Cell and Molecular Biology·Pablo PulidoFrancisco Javier Cejudo
Jan 22, 2005·Journal of Plant Physiology·José Luis Martínez-CamachoVictor Calderón-Salinas
Jul 31, 2001·Molecular Phylogenetics and Evolution·H GehrigM Kluge
Jul 16, 2013·Plant Science : an International Journal of Experimental Plant Biology·Ling HaoZhaohu Li
Aug 15, 2014·Frontiers in Plant Science·Fernando Domínguez, Francisco J Cejudo
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Veronika Doubnerová, Helena Ryšlavá
Mar 22, 2008·Journal of Experimental Botany·José A TraversoAna Chueca
May 22, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G BesnardB Offmann
Dec 31, 2003·The Journal of Biological Chemistry·Fernando DomínguezFrancisco Javier Cejudo

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