PMID: 11319148Apr 25, 2001Paper

Expression and molecular characterization of estrogen receptor alpha messenger RNA in male reproductive organs of adult goats

Biology of Reproduction
M M MansourH O Goyal

Abstract

The fact that male estrogen receptor alpha (ERalpha) knockout mice are infertile indicates a role for this receptor in male reproduction. Here, objectives were to evaluate ERalpha expression in male goat reproductive tissues at the transcriptional level using RNase protection assay (RPA) and in situ hybridization (ISH), and to clone a partial cDNA for caprine ERalpha using reverse transcription-polymerase chain reaction (RT-PCR). For RPA and ISH procedures, a radiolabeled antisense cRNA probe, generated in vitro from the ovine oER8 cDNA template, was employed. Evaluations were made on individual samples obtained from adult goats. Labeled cRNA sense probe was used as a negative control in ISH. A 530-base pair amplicon was generated by RT-PCR from efferent ductules (EDs), epididymis (EP), and testis, cloned from the ED and EP, and sequenced. The caprine ERalpha (cERalpha) cDNA displayed 81%-96% sequence identity with that of other species. A signal indicative of ERalpha mRNA was identified by both RT-PCR and RPA in all tissues, but was strongest in the ED. Compared with ED, ERalpha signal was sixfold lower in the EP, and 66-fold lower in the testis. Similarly, strong ERalpha expression was observed in ED epithelium, whereas littl...Continue Reading

References

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Citations

Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge Carreau, Rex A Hess
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Cleida A OliveiraRex A Hess
May 26, 2017·Physiological Reviews·Paul S CookeRex A Hess
Dec 31, 2004·Molecular Reproduction and Development·Saban TekinPeter J Hansen

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