Expression and phorbol ester-induced down-regulation of protein kinase C isozymes in osteoblasts

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
J Sanders, P H Stern

Abstract

The protein kinase C (PKC) enzyme family consists of at least 11 isozymes in three classes, with characteristic tissue distributions. Phorbol esters activate and ultimately down-regulate phorbol-sensitive isozymes. PKC is a signal transducer in bone, and phorbol esters influence bone resorption. Little is known about specific PKC isozymes in this tissue, however. We describe here the expression and phorbol ester-induced down-regulation of PKC isozymes in osteoblasts. Normal mouse osteoblasts and seven osteoblastic cell lines (rat UMR-106, ROS 17/2.8, ROS 24/1, and human MG-63, G-292, SaOS-2, HOS-TE85) were screened for isozyme expression by Western immunoblotting using isozyme-specific anti-PKC antibodies. The conventional alpha and beta I isozymes, but not gamma, were present in each of the osteoblasts examined; PKC-beta II was detectable in all but the ROS 24/1 line. PKC-epsilon was expressed in all osteoblasts screened, but other novel PKCs, delta, eta, and theta, were detectable only in select lines. The atypical zeta and iota/lambda PKCs were in all osteoblasts examined. To determine the sensitivity of the isozymes to prolonged phorbol ester treatment, normal osteoblasts and the UMR-106 cell line were treated with vehicle ...Continue Reading

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Citations

Mar 26, 2002·Molecular and Cellular Endocrinology·Moisés A Rivera-BermúdezWilliam S Mellon
Nov 14, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Wei-Kai HuaWey-Jinq Lin
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