Expression and potential role of microRNA-29b in mouse early embryo development.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Junqiang ZhangChang Liu

Abstract

MicroRNA-29b (miR29b) has been previously identified in early mouse embryos through miRNA microarray analysis. Recent research has indicated that miR29b participates in DNA methylation by regulating DNA methyltransferase 3a/3b (Dnmt3a/3b) expression. However, the expression pattern and biological function of miR29b in mouse preimplantation embryonic development remain unknown. In this study, we examined the expression patterns of miR29b and Dnmt3a/3b in mouse early embryos at different developmental stages. Subsequently, expression and localization of DNMT3A/3B protein was analyzed in mouse early embryos by immunofluorescence staining. The biological function of miR29b in mouse early embryos was analyzed by microinjection of commercially available miRNA-specific inhibitors and mimics. Our data showed that Dnmt3a/3b mRNA expression is negatively regulated by miR29b in mouse early embryos. Immunofluorescence analysis revealed that DNMT3A/3B protein expression is predominantly localized within the nucleoplasm of embryos. Alterations to the activity of miR29b could change the DNA methylation levels in mouse preimplantation embryos and lead to a developmental blockade, from the morula to the blastocyst stage. These results indicated...Continue Reading

Citations

Oct 18, 2016·Stem Cells and Development·Laila NoliDusko Ilic
Feb 22, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hong-Xia ChenXiao-Dong Zhou
Nov 23, 2017·Reproductive Biology and Endocrinology : RB&E·Jingjie LiangZhengguang Wang
Jul 1, 2016·Clinical and Experimental Reproductive Medicine·Neha BunkarPradyumna Kumar Mishra
Feb 28, 2018·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Shuang LiangXiang-Shun Cui
Oct 7, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas R HansenThomas E Spencer
Dec 19, 2017·The Journal of Reproduction and Development·Ying LinLi Chen
Jun 6, 2018·Frontiers in Cell and Developmental Biology·Alexandre ChamprouxAyhan Kocer
Dec 10, 2020·Animals : an Open Access Journal From MDPI·Ahmed GadRadek Procházka
Jul 14, 2021·Reproductive Sciences·J ShakerzadehK Parivar

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