Expression and Presence of OPG and RANKL mRNA and Protein in Human Periodontal Ligament with Orthodontic Force

Gene Regulation and Systems Biology
Liliana OteroLiseth Wilches-Buitrago

Abstract

The objective of this study is to investigate the expression and concentration of ligand receptor activator of NFkB (RANKL) and osteoprotegerin (OPG) in human periodontal ligament (hPDL) with orthodontic forces of different magnitudes. Right premolars in 32 patients were loaded with 4oz or 7oz of orthodontic force for 7 days. Left first premolars were not loaded. After 7 days, premolars were extracted for treatment as indicated. OPG and RANKL mRNA expressions were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and ELISA was used to assess OPG and RANKL protein concentration in compression and tension sides of PDL. Data were subjected to analysis of variance and Tukey tests. There was statistically significant difference in RANKL concentration on comparing control teeth with tension and compression sides of the experimental teeth (P < 0.0001). The expression of mRNA RANKL was increased in the tension and compression sides with 4oz (P < 0.0001). OPG did not show statistically significant association with any group. Changes in RANKL/OPG protein ratio in experimental and control groups showed statistically significant difference (P < 0.0001). RANKL protein levels are elevated in hPDL loaded with...Continue Reading

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Citations

Mar 11, 2020·Journal of Periodontal Research·Yoichi AritaRyuji Sakagami
Nov 10, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jian Zhou, Yimin Zhao
May 1, 2021·Journal of Clinical Medicine·Hyeran Helen JeonAndrew Tsai

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Methods Mentioned

BETA
fluorescence spectroscopy
ELISA
PCR
ELISAs

Software Mentioned

GraphPad Prism

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