PMID: 7027263Jul 1, 1981Paper

Expression and processing of bacterial beta-lactamase in the yeast Saccharomyces cerevisiae

Proceedings of the National Academy of Sciences of the United States of America
R RoggenkampC P Hollenberg

Abstract

The mode of expression in Saccharomyces cerevisiae of the bacterial antibiotic resistance gene coding for beta-lactamase (EC 3.5.2.6) is described. Yeast transformants, containing hybrid plasmid pMP78-1 consisting of pBR325 in a 2-micrometers DNA vector, synthesize an active beta-lactamase protein. The enzyme was purified about 100-fold over crude extracts. With regard to activity, molecular weight, and binding to specific antibodies the yeast beta-lactamase was indistinguishable from the purified enzyme from Escherichia coli. Because the bacterial enzyme is synthesized as a preprotein with subsequent maturation, the results suggest that S. cerevisiae is able to convert the preprotein to the mature beta-lactamase. This was confirmed by in vitro experiments showing that the bacterial preprotein can be processed by crude extracts of S. cerevisiae.

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R P Ambler, G K Scott
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J D CohenJ Marmur
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert

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Citations

Dec 1, 1982·Current Genetics·G ReipenC P Hollenberg
Jan 1, 1983·The International Journal of Biochemistry·I Suominen, P Mäntsälä
Dec 1, 1982·Gene·Z A JanowiczC P Hollenberg
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K HylandR Baserga
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Müller, G Blobel
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S J RothsteinJ C Gray
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I T Fecycz, G Blobel
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G ZambettiG Stein
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·S M KingsmanN A Roberts
Feb 5, 2008·Applied and Environmental Microbiology·Pedro M R GuimarãesLucília Domingues
Jun 14, 1984·Nature·M WiedmannT A Rapoport
Mar 1, 1988·Molecular Microbiology·O PinesM Inouye
Jun 1, 1992·Yeast·M A RomanosJ J Clare
Nov 30, 2012·Biopolymers·Antônio Américo Barbosa VianaMaria Fátima Grossi-de-Sá
Sep 1, 1983·Microbiological Reviews·T J SilhavyS D Emr
Dec 1, 1984·Microbiological Reviews·L L Randall, S J Hardy
Jul 1, 1987·Journal of Bacteriology·P Z WangR P Novick
Jan 1, 1983·Journal of Cellular Biochemistry·R RoggenkampC P Hollenberg

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