PMID: 9539105Apr 16, 1998Paper

Expression and processing of G0/G1 switch gene 24 (G0S24/TIS11/TTP/NUP475) RNA in cultured human blood mononuclear cells

DNA and Cell Biology
S P HeximerD R Forsdyke

Abstract

The human G0/G1 switch (G0S) gene, G0S24, and its rodent immediate-early homolog (TIS11, TTP, NUP475) are part of a mammalian gene family whose members encode CCCH zinc finger domains and domains similar to part of the large subunit of RNA polymerase II and to the Mei2 regulator of G1 arrest in fission yeast. We compared the RNA expression of G0S24 with that of other G0S genes in cultured blood mononuclear cells and examined the levels of various RNA processing intermediates. Freshly isolated cells contained high levels of several G0S RNAs, which declined by 24 h, suggesting transient spontaneous stimulation during cell purification (Heximer et al., 1996). However, in cells preincubated for 24 h, G0S24 RNA levels remained much higher than those of other G0S genes (107+/-42 x 10(6) molecules/microg of RNA); stimulation with lectin (Con-A) further increased G0S24 RNA, much of which remained nuclear. Like those of FOS/G0S7, EGR1/G0S30 and of the gene encoding the regulator of G protein signalling 1 (RGS1), G0S24 RNA levels increased more in response to a protein kinase C activator than to a calcium ionophore, whereas the opposite held for FOSB/G0S3 and RGS2/G0S8. With appropriate PCR primer pairs, we showed a G0S24 RNA processing ...Continue Reading

References

Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Aug 17, 1989·Nature·B S Baker
Jul 6, 1989·Nature·M SubramaniamM J Getz
Jun 28, 1985·Biochemical and Biophysical Research Communications·D R Forsdyke
Jul 10, 1982·Nucleic Acids Research·R Miesfeld, N Arnheim
Nov 1, 1993·Trends in Biochemical Sciences·W Nellen, C Lichtenstein
Jun 1, 1995·Current Opinion in Genetics & Development·S H Cross, A P Bird
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·R Evers, I Grummt
Jan 1, 1994·Annual Review of Biochemistry·G R Crabtree, N A Clipstone
May 1, 1993·Molecular and Cellular Biology·J T AndersonM S Swanson
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·M T WorthingtonJ M Berg
Dec 1, 1996·Molecular and Cellular Biology·C Bai, P P Tolias
Dec 1, 1996·DNA and Cell Biology·S P HeximerD R Forsdyke
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M Kumar, G G Carmichael
Jun 15, 1997·Nucleic Acids Research·S BrackenridgeN J Proudfoot
Jun 1, 1982·Molecular and Cellular Biology·M Salditt-Georgieff, J E Darnell

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Citations

Feb 13, 2001·Gene·D R Forsdyke, J R Mortimer
Dec 5, 2002·Trends in Immunology·Donald R ForsdykeScott D Smith
Aug 3, 2002·Immunology·Eun Sook Hwang, I-Cheng Ho
Aug 6, 2009·The Journal of Biological Chemistry·Yvonne M SchichlRainer de Martin
Oct 25, 2003·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Kelly L RogersLyn R Griffiths

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