Expression and purification of chicken beta interferon and its antivirus immunological activity

Protein Expression and Purification
Meihong CaiPingping Shen

Abstract

Interferon beta (IFN-β) belongs to a class of natural proteins that can inhibit virus replication. In this paper, nucleic acid sequence encoding chicken interferon beta (chIFN-β) mature protein was cloned and highly expressed in Escherichia coli (E. coli) by 1mM IPTG induction. The expressed chIFN-β was about 20% of total bacterial protein. The recombinant protein in form of inclusion body was then solubilised, refolded and purified to a purity of greater than 95% by immobilized metal ion affinity chromatography (IMAC). This recombinant chIFN-β could inhibit vesicular stomatitis Indiana virus (VSV) and infectious bursal disease virus (IBDV) replication in vitro. Animal experiments showed that the recombinant chIFN-β could decrease pathological lesions caused by the IBDV in bursa of Fabricius. These results will provide useful information for further investigations on veterinary clinical applications and fundamental research.

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Citations

Mar 29, 2013·Fish & Shellfish Immunology·Shousheng LiuShicui Zhang
Mar 16, 2018·Journal of Virology·Liliana L Cubas-GaonaDolores Rodríguez
Jul 25, 2021·Developmental and Comparative Immunology·Congjun LiGuangdong Ji

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