Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216

Protein Expression and Purification
Hui-Ting SongLi-Xin Ma

Abstract

We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized.

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Citations

Aug 3, 2010·Applied Microbiology and Biotechnology·Wen-Shan LiuYun-Jun Yan
Oct 15, 2009·Journal of Biomedicine & Biotechnology·Farshad DarvishiFariborz Momenbeik
Jun 9, 2012·Microbial Cell Factories·Víctor Barba CedilloMaría Jesús Martínez
Feb 18, 2011·New Biotechnology·Farshad DarvishiHamid Zarkesh-Esfahani
Aug 22, 2007·Protein Expression and Purification·Zheng-Bing JiangZhen-Bin Wu
Nov 29, 2013·Biotechnology and Bioengineering·Hyun Tae HwangArvind Varma
Sep 6, 2007·FEMS Yeast Research·Pascale JolivetJean Marc Nicaud
Jul 24, 2021·International Journal of Biological Macromolecules·Abdallah R IsmailKwang-Hyun Baek

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