Expression and regulation of carbonic anhydrases in the marine diatom Thalassiosira pseudonana and in natural phytoplankton assemblages from Great Bay, New Jersey

Physiologia Plantarum
Patrick J McGinn, François M M Morel

Abstract

BLAST searches of expressed sequence tag libraries have revealed putative homologs of the archetypal diatom delta-carbonic anhydrase (CA) TWCA1 (for Thalassiosira weissflogii CA) in a broad range of eukaryotic phytoplankton including haptophytes, prasinophytes and dinoflagellates. Four putative homologs of TWCA1 are also reported and described from a search of the genomic sequence of Thalassiosira pseudonana and designated TWCA(Tp1-4). The delta-CA class is therefore more widely distributed in marine phytoplankton than previously thought. In particular, it is not restricted to the diatoms like the cadmium-containing enzyme, CDCA, seems to be (zeta-CA class). Zinc status strongly influences growth rate in marine diatoms. We observed a decrease in the specific growth rate of T. pseudonana from 2.1 to 1.3 day(-1) when the unchelated Zn concentration (Zn') was lowered from 20 to 5 pM. In the same cultures, we detected a drop in whole-cell CA activity of about 50% in the most Zn-limited cells that occurred simultaneously with a large downregulation of TWCA(Tp1), TWCA(Tp2) and CDCA(Tp) gene transcripts and protein. These three genes were also found to be strongly upregulated by low pCO(2) in a manner typical of many algal CA enzymes....Continue Reading

References

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Citations

May 4, 2010·Applied and Environmental Microbiology·Eve ToulzaDaniel Grzebyk
Jan 15, 2014·Photosynthesis Research·Mio SamukawaYusuke Matsuda
Feb 19, 2011·Annual Review of Marine Science·John R Reinfelder
Feb 16, 2016·Scientific Reports·Feng-Jiao LiuXu-Guang Huang
Apr 15, 2008·Physiologia Plantarum·Francisco J L Gordillo
Sep 23, 2014·Journal of Plant Physiology·Peter G Kroth
Mar 27, 2018·Journal of Enzyme Inhibition and Medicinal Chemistry·Silvia BuaClaudiu T Supuran
Jun 5, 2010·Photosynthesis Research·John A Raven
Apr 4, 2017·Journal of Experimental Botany·Romain ClementBrigitte Gontero
Apr 26, 2020·International Journal of Molecular Sciences·Erik L JensenBrigitte Gontero
May 17, 2014·Metallomics : Integrated Biometal Science·Rachel Narehood Austin, Mak A Saito

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