PMID: 8947601Nov 1, 1996Paper

Expression and regulation of constitutive and acute phase serum amyloid A mRNAs in hepatic and non-hepatic cell lines

Scandinavian Journal of Immunology
D M SteelAlexander S Whitehead

Abstract

'Acute phase' and 'constitutive' SAA (A-SAA and C-SAA, respectively) mRNA levels were measured in hepatic and non-hepatic cell lines after treatment with monocyte conditioned medium (MoCM), with or without dexamethasone (Dex). A-SAA mRNAs were detected in MoCM-treated hepatoma cell lines (PLC/PRF/5, HuH7, HepG2, and Hep3B), a fibroblast cell line (MRC5), six epithelial cell lines (RT4/ 31, SW13, Hela Ohio, HCT-8, CaCo2, and KB), and an endothelial cell line ECV304. In KB cells, Dex alone caused a dramatic increase in A-SAA mRNA levels. C-SAA was detected in all hepatic and non-hepatic cell lines. Two differentially regulated size classes of C-SAA mRNA were detected in the hepatoma cell lines. A-SAA mRNA levels were measured in ECV304 cells treated with IL-1 beta, IL-6, TNF alpha and Dex, in various combinations, and revealed different profiles to those seen for hepatic cells. The extent of polyadenylation of A-SAA mRNA in ECV304 and KB cells differed whereas the polyadenylation of C-SAA mRNA remained constant. These data suggest that the parameters that determine the steady state mRNA levels and post-transcriptional regulation of A-SAA and C-SAA mRNAs are different and are cell type specific.

Citations

Feb 21, 2008·Cancer Immunology, Immunotherapy : CII·Raffaele Adolfo CalogeroFederica Cavallo
Dec 4, 2001·Veterinary Immunology and Immunopathology·T L McDonaldA Weber
Dec 23, 1999·Current Opinion in Hematology·S Urieli-ShovalY Matzner
Jan 11, 2007·Journal of Burn Care & Research : Official Publication of the American Burn Association·Timothy P PlackettElizabeth J Kovacs
Nov 17, 2007·American Journal of Respiratory and Critical Care Medicine·Steven BozinovskiGary P Anderson
Feb 23, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Kendal HarrRuth Francis-Floyd
Jan 23, 2016·Cytokine & Growth Factor Reviews·Mieke De BuckJo Van Damme
May 21, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Satoru ShinrikiYukio Ando
Jun 28, 2011·American Journal of Ophthalmology·Yan MaYan-Rong Jiang
Jun 3, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Barbara ZeregaFiorella Descalzi Cancedda
Dec 17, 2008·Fish & Shellfish Immunology·Berglind GisladottirBergljot Magnadottir
Aug 24, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Orit GutfeldSimcha Urieli-Shoval
Nov 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S Urieli-ShovalY Matzner
Mar 8, 2018·FEBS Open Bio·George H SackThomas L McDonald
Oct 3, 1999·European Journal of Biochemistry·C M Uhlar, A S Whitehead
Mar 7, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Nancy R WebbFrederick C De Beer
Jan 22, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Xiao-Chun NiShuang-Jian Qiu
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D YangJ J Oppenheim
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caroline F Thorn, Alexander S Whitehead
Feb 29, 2020·Research and Practice in Thrombosis and Haemostasis·José A FernándezJohn H Griffin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Arnold S MontoMatthew Sarnes
© 2021 Meta ULC. All rights reserved