Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment

Scientific Reports
Qingbin ZhangJian Ren

Abstract

Although long noncoding RNAs (lncRNAs) have been emerging as critical regulators in various tissues and biological processes, little is known about their expression and regulation during the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in inflammatory microenvironment. In this study, we have identified 63 lncRNAs that are not annotated in previous database. These novel lncRNAs were not randomly located in the genome but preferentially located near protein-coding genes related to particular functions and diseases, such as stem cell maintenance and differentiation, development disorders and inflammatory diseases. Moreover, we have identified 650 differentially expressed lncRNAs among different subsets of PDLSCs. Pathway enrichment analysis for neighboring protein-coding genes of these differentially expressed lncRNAs revealed stem cell differentiation related functions. Many of these differentially expressed lncRNAs function as competing endogenous RNAs that regulate protein-coding transcripts through competing shared miRNAs.

References

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Citations

Nov 28, 2018·Journal of Biomedical Materials Research. Part a·Fei XuHong Zheng
Aug 28, 2019·Journal of Cellular Biochemistry·Ruhai YiYueqing Su
Jan 30, 2021·Neural Regeneration Research·Viviana Nociti, Massimo Santoro
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Min ZhaoZhe-Sheng Chen
Jul 24, 2021·Journal of Periodontal Research·Jingchen XuSong Chen

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Datasets Mentioned

BETA
SRP096798

Methods Mentioned

BETA
RNA-seq
PCA
Protein Assay
electrophoresis

Software Mentioned

miRanda
PLEK
RSEM
STAR
gffread
cufflinks
LncRNAs2Pathway
EdgeR
cuffmerge
Lncipedia

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