Expression and regulation of M-type K+ channel in PC12 cells and rat adrenal medullary cells

Cell and Tissue Research
K HaradaM Inoue

Abstract

M-type K+ channels contribute to the resting membrane potential in the sympathetic ganglion neurons of various animals, whereas their expression in adrenal medullary (AM) cells has been controversial. The present experiment aims to explore the expression of M channels comprising the KCNQ2 subunit in the rat AM cell and its immortalized cell line PC12 cells at the protein level and how its expression in PC12 cells is regulated. The KCNQ2 isoform was recognized in homogenates of PC12 cells but not the rat adrenal medullae by immunoblotting and KCNQ2-like immunoreactivity (IR) was detected in PC12 cells but not in rat AM cells. When the PC12 cells were maintained in a dexamethasone-containing medium, KCNQ2-like IR in the cells was suppressed, whereas the removal of fetal bovine serum from the culture medium for 1 day resulted in an increase in KCNQ2-like IR. A similar enhancement occurred when PC12 cells were cultured under conditions where glucocorticoid receptor (GR) and/or mineralocorticoid receptor (MR) activities were suppressed. These morphological findings were confirmed in functional analysis. The cells cultured in the presence of an inhibitor of either GR or MR exhibited larger amplitudes of Ca2+ signal in response to an ...Continue Reading

References

Feb 1, 1990·The Journal of Physiology·J I Medbø, O M Sejersted
Dec 1, 1993·The Journal of Physiology·T ClausenJ A Flatman
Jan 1, 1997·Annual Review of Medicine·F CadepondE E Baulieu
May 3, 2002·The Journal of Physiology·Damian J WallacePhilip D Marley
Oct 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Omar A ItaniChristie P Thomas
Sep 25, 2003·Physiological Reviews·Torben Clausen
Nov 19, 2005·Endocrinology·Celso E Gomez-SanchezElise P Gomez-Sanchez
Nov 13, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ainhoa EtxeberriaAlvaro Villarroel
Jun 5, 2008·Nature Reviews. Molecular Cell Biology·Tatjana Sauka-Spengler, Marianne Bronner-Fraser
Mar 6, 2009·American Journal of Physiology. Renal Physiology·Joel C Geerling, Arthur D Loewy
Mar 21, 2009·British Journal of Pharmacology·David A Brown, Gayle M Passmore
Jun 10, 2010·The Journal of Physiology·Florian LangUndine E Lang
May 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jie ZhangMark S Shapiro
Nov 19, 2011·Journal of Pharmacological Sciences·Keita HaradaMasumi Inoue
Feb 16, 2012·The Journal of Biological Chemistry·Andrew P StewartJ Michael Edwardson
May 25, 2012·Endocrinology·Cristina Aguilar-SánchezDiego Alvarez de la Rosa
Jun 30, 2012·American Journal of Physiology. Cell Physiology·Masumi InoueAkira Warashina
Feb 9, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pablo MirandaTeresa Giraldez
Aug 21, 2013·Trends in Pharmacological Sciences·Mahita Kadmiel, John A Cidlowski
Oct 3, 2013·The Journal of Allergy and Clinical Immunology·Robert H Oakley, John A Cidlowski
May 5, 2017·Endocrinology·Maniselvan KuppusamyCelso E Gomez-Sanchez
May 12, 2017·The Journal of Endocrinology·James S M CuffeVicki L Clifton
Aug 2, 2017·Pflügers Archiv : European journal of physiology·Masumi InoueLung-Sen Kao

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Citations

Jul 13, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Masumi InoueKeita Harada
Mar 12, 2021·Journal of Neurochemistry·Keita HaradaMasumi Inoue

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Methods Mentioned

BETA
protein assay
transfect
proximity ligation assay

Software Mentioned

HCImage
NIH image software

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