PMID: 9662704Jul 15, 1998Paper

Expression and regulation of mitochondrial uncoupling protein 1 from brown adipose tissue in Leishmania major promastigotes

Molecular and Biochemical Parasitology
E Alvarez-FortesLuis Rivas

Abstract

Rat uncoupling protein 1 (UCP1) was successfully translated in transfected Leishmania major promastigotes. Immune electron microscopy revealed that the protein was exclusively in the mitochondria. UCP1 expression was about 350,000 copies per promastigote, accounting for 4.7% of the total mitochondrial protein. In intact parasites, expression of UCP1 induced a slight increase in respiratory rate and a modest decrease in mitochondrial membrane potential (delta psi(m)). In contrast, in digitonin-permeabilized parasites, a significantly lower value both in delta psi(m) (57 +/- 10 vs 153 +/- 12 mV) and respiratory control ratio (0.99 vs 1.54) were observed for UCP1 versus control parasites, although when UCP1 activity was inhibited by bovine serum albumin (BSA) and GDP, control values were restored. Therefore, a fully functional UCP1 was present and only partially inhibited in vivo by endogenous purine nucleotides. However, neither ATP levels, growth rate nor mitochondrial protein import differed significantly between both types of parasites. Expression of the pore-like mutant UCP1 delta 9 was deleterious to the organism. Consequently, Leishmania was capable of expressing and importing into mitochondria proteins from higher eukaryot...Continue Reading

References

Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R S Kutzman, J F Roberts
Oct 1, 1976·FEBS Letters·K E Akerman, M K Wikström
Dec 1, 1992·The Journal of Antimicrobial Chemotherapy·S L CroftA W Randall
Aug 1, 1989·Canadian Journal of Physiology and Pharmacology·P Trayhurn, R E Milner
Dec 1, 1982·Experimental Parasitology·D T Hart, G H Coombs
Nov 1, 1981·Molecular and Biochemical Parasitology·D T HartG H Coombs
Sep 1, 1995·Microbiological Reviews·C ClaytonJ Blattner
Jan 1, 1994·Methods in Cell Biology·J H LeBowitz
Oct 1, 1993·Journal of Bioenergetics and Biomembranes·J E Walker, M J Runswick
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A P FernandesS M Beverley
Dec 15, 1993·The Biochemical Journal·I ArechagaE Rial
May 1, 1993·The Journal of Eukaryotic Microbiology·A A DivoA C Sartorelli
Dec 1, 1995·Antimicrobial Agents and Chemotherapy·L ZhaiA Kharazmi
Dec 1, 1995·Molecular and Biochemical Parasitology·K R Santhamma, A Bhaduri
Aug 1, 1996·Current Opinion in Cell Biology·R LillW Neupert
Mar 1, 1997·Molecular and Biochemical Parasitology·J J Van Hellemond, A G Tielens
Sep 20, 1997·Nature·M LaloiD Ricquier
Jul 17, 1998·Current Opinion in Chemical Biology·K StuartS Lawson
Jan 1, 1994·Parasitology Today·A G Tielens

❮ Previous
Next ❯

Citations

Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Trevor Lithgow, André Schneider
Sep 15, 2010·Antimicrobial Agents and Chemotherapy·Luis CarvalhoFrancisco Gamarro
Feb 7, 2007·Antimicrobial Agents and Chemotherapy·Juan Román Luque-Ortega, Luis Rivas
Dec 11, 2007·Trends in Cell Biology·André SchneiderTrevor Lithgow
Dec 10, 2014·Antimicrobial Agents and Chemotherapy·María Ángeles AbengózarLuis Rivas
Jan 31, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan Román Luque-OrtegaLuis Rivas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.