PMID: 9555880Apr 29, 1998Paper

Expression and regulation of the sodF gene encoding iron- and zinc-containing superoxide dismutase in Streptomyces coelicolor Müller

Journal of Bacteriology
E J KimJ H Roe

Abstract

Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved among known FeSODs and MnSODs. The deduced amino acid sequence exhibited highest similarity to Mn- and FeSODs from Propionibacterium shermanii and Mycobacterium spp. The transcription start site of the sodF gene was determined by primer extension. When the sodF gene was cloned in pIJ702 and introduced into Streptomyces lividans TK24, it produced at least 30 times more FeZnSOD than the control cells. We disrupted the sodF gene in S. lividans TK24 and found that the disruptant did not produce any FeZnSOD enzyme activity but produced more NiSOD. The expression of the cloned sodF gene in TK24 cells was repressed significantly by Ni, consistent with the regulation pattern in nonoverproducing cells. This finding suggests that the cloned sodF gene contains the cis-acting elements necessary for Ni regulation. When the sodF mRNA in S. coelicolor Muller cells was analyzed by S1 mapping of both 5' and 3' ends, we found that Ni ...Continue Reading

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Citations

Feb 17, 2007·Journal of Basic Microbiology·Astrid SchmidtErika Kothe
Jan 12, 2011·The Protein Journal·Yuanyuan ZouZhifang Zhang
Mar 3, 2009·Journal of Basic Microbiology·Astrid SchmidtErika Kothe
Aug 12, 2008·Chembiochem : a European Journal of Chemical Biology·Matthias SchmidtJames Weston
Dec 20, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ylenia BeniaminoBarbara Zambelli
Aug 29, 2009·Chemical Reviews·Yanjie Li, Deborah B Zamble
May 5, 1999·Current Opinion in Chemical Biology·M J Maroney
Jan 25, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·O DussurgetI Smith
Nov 28, 2012·Journal of Proteome Research·Eva StrakovaJiri Vohradsky
Feb 7, 2008·Biochimica Et Biophysica Acta·Chris D den Hengst, Mark J Buttner

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