Expression and release of the latent transforming growth factor beta binding protein by hepatocytes from rat liver

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
S RothA M Gressner

Abstract

In very recent studies it was established that transforming growth factor beta (TGF-beta), likely to be the most relevant fibrogenic cytokine and regulator of cell proliferation, differentiation, and matrix metabolism, is expressed by hepatocytes (parenchymal cell [PC]) and secreted from cultured PC in a latent form incapable of receptor binding. The structural composition of the latent TGF-beta complex secreted by cultured PC is unknown. In some TGF-beta expressing cell types this cytokine is released as a large molecular weight complex containing in addition to the TGF-beta latency associated peptide (LAP) a disulfide bonded latent TGF-beta binding protein (LTBP), of which the existence and function in liver is hitherto unknown. This study is directed to the identification of LTBP expression in rat PC. Cells were isolated from rat liver with the collagenase method and analyzed for LTBP before and during culture under standard conditions using alkaline phosphatase anti-alkaline phosphatase (APAAP) immunostainings, metabolic labeling, messenger RNA (mRNA) detection (reverse-transcription polymerase chain reaction [RT-PCR]) and sequencing, and immunoblotting of gel chromatographically separated cell extracts and conditioned medi...Continue Reading

Citations

Apr 16, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S RothA M Gressner
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H C Pitot
May 28, 2015·BMC Gastroenterology·Alexandra I ThompsonNeil C Henderson
Jun 21, 2011·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Ghada M GalalMona A H El-Baz
Aug 30, 2016·World Journal of Gastroenterology : WJG·Norio KuboKen Shirabe

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