PMID: 7536058Apr 1, 1994Paper

Expression and role of c-myc protooncogene in murine preimplantation embryonic development

Journal of Assisted Reproduction and Genetics
R K NazB S Minhas

Abstract

The present study was conducted to investigate the expression and possible role of the c-myc protooncogene in preimplantation embryos by using reverse-transcriptase/polymerase chain reaction (RT-PCR) technique and microinjection of synthetic antisense c-myc oligonucleotide probe, respectively. Total RNA was extracted from oocytes and two cell-, four cell-, early morula-, late morula-, early blastocyst-, and late blastocyst-stage embryos, and cDNA was constructed using MMLV reverse transcriptase. Sense primer (P1) and antisense primer (P2) used were based on the c-myc gene sequence bp 1609-1629 and bp 3279-3299, respectively, that span a 1.37-kb intron. PCR-amplified products of cDNA from oocyte-, two cell-, four cell-, early and late morula-, and blastocyst-stage embryos demonstrated the expected 313-bp product in Southern blot hybridization using a c-myc specific DNA probe, with an indication of lower levels in oocytes and early morulae. Cytoplasmic injection of the antisense c-myc oligonucleotide probe (P2) and not the sense probe (P1) into pronuclear-stage zygotes caused a significant (P = 0.02 to 0.0001) inhibition of development to blastocysts in a concentration-dependent manner, with a maximal inhibition at the first clea...Continue Reading

References

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May 1, 1992·Developmental Biology·R J ArceciJ W Pollard
Jan 1, 1992·Biology of Reproduction·R K Naz
Jan 25, 1991·Cell·L C CantleyS Soltoff
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Citations

Nov 19, 1997·Molecular Reproduction and Development·R K Naz, X Zhu
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·R E LeachD R Armant
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·X Zhu, R K Naz
Jan 4, 2012·Molecular Reproduction and Development·Lucie MarandelPierre-Yves Le Bail
Aug 16, 2005·Biochemical and Biophysical Research Communications·Yanhong GaoXuetao Pei
Jul 9, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·W L GuoJ Wang
May 1, 1997·Molecular Reproduction and Development·A D DomashenkoK S Hatton
Jun 1, 1997·Molecular Reproduction and Development·R K NazA C Menge
Feb 7, 2008·Reproductive Biomedicine Online·Stephen SullivanHidenori Akutsu

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