PMID: 15236606Jul 9, 2004Paper

Expression and role of integrins in invasive activity of oncotransformed fibroblasts differing in spontaneous metastasizing

Biochemistry. Biokhimii︠a︡
Galina E MorozevichAlbert E Berman

Abstract

Four closely related lines of RSV-transformed Syrian hamster fibroblasts differing drastically in their spontaneous metastatic capacity were investigated for the surface expression of integrins, in vitro invasion, and production of MMP-2 collagenase. The highly metastasizing HET-SR-2SC-LNM cells differ from the lowly metastasizing parental HET-SR cells in a high level of the surface expression of the collagen-specific alpha1beta1, alpha2beta1, and alphavbeta3 integrins, a high invasive activity, and an increased production of MMP-2. The same properties are characteristic for the actively metastasizing cells of the independent HET-SR-1 line. The lowly metastasizing fibroblasts that are derived from HET-SR-2SC-LNM retain a high level of the expression of the alpha1beta1 and alpha2beta1 integrins, but, unlike the parental line, they exhibit a decreased expression of the alphavbeta3 integrin, invasion in Matrigel, and MMP-2 production. Substrate stimulation of the signal function of the collagen-specific integrins increases the production of MMP-2 by the metastatically active fibroblasts. Inhibition of the signal activity of the integrins by RGD-containing pentapeptide or by genistein reduces markedly in vitro invasion in Matrigel ...Continue Reading

Citations

Aug 31, 2013·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A E BermanG E Morozevich
Apr 27, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·G E MorozevichA E Berman

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