Expression and role of nestin in human cervical intraepithelial neoplasia and cervical cancer.

International Journal of Oncology
Atsuki SatoZenya Naito

Abstract

Nestin expression reportedly correlates with aggressive growth, metastasis, poor prognosis and presence of cancer stem cells (CSCs) in various tumors. In this study, we determined the expression and role of nestin in cervical intraepithelial neoplasia (CIN) and cervical cancer. We performed immunohistochemical and in situ hybridization analyses of nestin in 26 cases for each stage of CIN and 55 cervical cancer tissue samples. To examine the role of nestin in cervical cancer cells, we stably transfected expression vectors containing nestin cDNA into ME-180 cells. We studied the effects of increased nestin expression on cell proliferation, cell motility, invasion as well as sphere and soft agar formation. Nestin was not localized in the squamous epithelium in normal cervical tissues, but it was weakly expressed in the basal squamous epithelium of CIN 1. In CIN 2, nestin was localized to the basal to lower 2/3 of the squamous epithelium, whereas in CIN 3, it was localized to the majority of the squamous epithelium. Nestin was detected in all cases of invasive cervical cancer. N...Continue Reading

References

Jul 1, 1994·Journal of Neuropathology and Experimental Neurology·G SjöbergT Sejersen
Sep 1, 1994·Developmental Biology·A M KachinskyJ B Miller
Jul 1, 1996·Acta Neuropathologica·T TakanoL E Becker
Apr 1, 1995·Neurobiology of Disease·R C LinO Brüstle
Jan 1, 1997·The European Journal of Neuroscience·S HolminT Mathiesen
May 1, 1997·Differentiation; Research in Biological Diversity·K FröjdmanJ E Eriksson
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T NikiA Geerts
Jun 8, 2000·Mechanisms of Development·J YangN Jing
Mar 29, 2001·Microscopy Research and Technique·G AlmazánC Guaza
Jul 11, 2002·Histochemistry and Cell Biology·Jessy LardonLuc Bouwens
Jun 24, 2004·Neuromuscular Disorders : NMD·Ville AärimaaHannu Kalimo
Aug 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki AmohRobert M Hoffman
Sep 21, 2004·Oncogene·Sheila K SinghPeter B Dirks
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·C WieseA M Wobus
Dec 5, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Walter M KleinSteven R Tahan
Apr 24, 2007·Journal of Cutaneous Pathology·Svetlana BrychtovaJaroslav Hirnak
Jul 6, 2007·Journal of Neuro-oncology·Yue-Hui MaJanka Held-Feindt
May 31, 2008·Archivum Immunologiae Et Therapiae Experimentalis·Sabine Hombach-KlonischMarek Los
Jan 29, 2009·Molecular Cancer Therapeutics·Antonio JimenoManuel Hidalgo
Mar 10, 2009·Cancer Letters·Hiroya YamadaAkira Miyauchi
Dec 24, 2009·Cancer Science·Caigang LiuPing Lu
Jan 1, 2010·Oncology Reports·Kazuto OkunoYoshihiro Takeuchi
Jul 8, 2010·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Keokedthong PhongsavanLena Marions
Jul 9, 2010·The Cochrane Database of Systematic Reviews·Xiaohu WangHong Yin
Nov 4, 2010·Nucleic Acids Research·Subhash M AgarwalG P S Raghava
Jan 25, 2011·Cancer Biology & Therapy·Yoko MatsudaToshiyuki Ishiwata

❮ Previous
Next ❯

Citations

Oct 24, 2013·Pathology International·Hiroyuki YanaiTadashi Yoshino
May 3, 2015·BMC Cancer·Ariane GiannattasioJoachim Koch
Sep 14, 2019·Oncology Letters·Gretel Mendoza-AlmanzaJorge Olmos
Jul 17, 2020·Frontiers in Cellular and Infection Microbiology·Megha BudhwaniJanin Chandra
Dec 21, 2016·The Journal of Obstetrics and Gynaecology Research·Veysel SalHafize Uzun
Feb 26, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rajasree Sreedharan, Scott K Van Why
Apr 5, 2013·Molecular Cancer Research : MCR·Huei-Ting SuKuang-Hung Cheng
Jun 1, 2021·Veterinary Pathology·Hisashi YoshimuraToshiyuki Ishiwata
Aug 29, 2021·Cancer Metastasis Reviews·Kalyani PatilShahab Uddin

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
transfection
PCR
ELISA

Software Mentioned

Stat View J
Metamorph
- C1
EZ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.