PMID: 8589054Jan 1, 1995Paper

Expression and secretion of malarial parasite beta-tubulin in Bacillus brevis

Biochimie
A BellR M Franklin

Abstract

Microtubule inhibitors are active against the human malarial parasite Plasmodium falciparum, but whether these drugs actually interact with parasite tubulins is not known. It has not previously been possible to produce mg quantities of isolated, soluble tubulin subunits for drug-binding experiments. A cDNA encoding P falciparum beta-tubulin was expressed and the protein secreted in Bacillus brevis. With the addition of EGTA to the culture medium, which increases shedding of proteins from the cell surface, up to 2 mg/l recombinant beta-tubulin was obtained in supernatants. It is not clear why B brevis is able to secrete this normally cytoplasmic protein, but the secretion levels of recombinant proteins may be related to the net charge of the first few residues of the mature polypeptide.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1992·Cell Motility and the Cytoskeleton·J C Zabala, N J Cowan
Dec 1, 1990·Applied Microbiology and Biotechnology·H KonishiS Udaka
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H Andersson, G von Heijne
May 1, 1989·Cell Biology International Reports·A Dieckmann-Schuppert, R M Franklin
Jan 1, 1989·Biotechnology & Genetic Engineering Reviews·S UdakaH Yamagata
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H YamagataS Udaka
Mar 22, 1988·Biochemistry·M B YaffeH Sternlicht
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LiH Inouye
Nov 20, 1986·Journal of Molecular Biology·G von Heijne
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D M HelfmanS H Hughes
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva
Jan 1, 1993·Methods in Enzymology·S Udaka, H Yamagata
Jan 1, 1993·Parasitology Research·A BellR M Franklin

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Citations

Aug 9, 2003·International Journal for Parasitology·Clare S GaviganAngus Bell
Oct 17, 2006·Parasitology Today·A Bell
Aug 9, 2007·Future Microbiology·Barbara Kappes, Petra Rohrbach
Sep 26, 2001·Protein Expression and Purification·K YashiroR J Moore

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