Expression and secretion of Salmonella pathogenicity island-2 virulence genes in response to acidification exhibit differential requirements of a functional type III secretion apparatus and SsaL.

The Journal of Biological Chemistry
Brian K CoombesB Brett Finlay

Abstract

Salmonella pathogenicity island (SPI)-2 is pivotal to the intracellular survival of Salmonella and for virulence in mammals. SPI-2 encodes virulence factors (called effectors) that are translocated into the host cell, a type III secretion apparatus and a two-component regulatory system that regulates intracellular expression of SPI-2. Salmonella SPI-2 secretion activity appears to be induced in response to acidification of the vacuole in which it replicates. Here we show that the expression of the SPI-2 proteins, SseB and SseD (filament and pore forming components of the secretion apparatus, respectively) in response to acidification requires an intact secretion system and SsaL, a Salmonella homologue of SepL, a regulator required for type III-dependent secretion of translocators but not effectors in attaching and effacing gastrointestinal pathogens. We show that the expression of SPI-2-encoded effectors is acid-regulated but can be uncoupled from the expression of filament and translocon components, thus showing a differential requirement of SsaL for expression. The secretion and translocation of SPI-2-encoded effectors requires SsaL, but SsaL is dispensable for the secretion of SPI-2 effectors encoded in other pathogenicity l...Continue Reading

References

Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E SheaD W Holden
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·H OchmanE A Groisman
Dec 1, 1996·Molecular Microbiology·F J Mojica, C F Higgins
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Sep 29, 1998·Methods in Enzymology·D F SenearS A Gavigan
Apr 21, 1999·Molecular Microbiology·J DeiwickM Hensel
Jul 16, 1999·The EMBO Journal·K UchiyaE A Groisman
Jun 9, 2000·Molecular Microbiology·M J WorleyF Heffron
Oct 29, 2000·Journal of Bacteriology·A U KresseC A Guzmán
Feb 24, 2001·Cellular Microbiology·J H BrumellB B Finlay
Feb 21, 2002·Infection and Immunity·Imke Hansen-WesterMichael Hensel
Feb 27, 2003·Molecular Microbiology·Daniel V Zurawski, Murry A Stein
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ariel BlockerShin-Ichi Aizawa
May 20, 2003·Molecular Microbiology·Xiuhong FengLinda J Kenney
Jun 6, 2003·Microbes and Infection·Brian K CoombesB Brett Finlay
Oct 1, 2003·The EMBO Journal·Suzana P Salcedo, David W Holden
Oct 24, 2003·Infection and Immunity·James B Day, Catherine A Lee
Feb 19, 2004·Journal of Cell Science·Julie GuignotDavid W Holden
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wanyin DengB Brett Finlay

❮ Previous
Next ❯

Citations

Mar 17, 2010·Nature Methods·Schuyler B Van Engelenburg, Amy E Palmer
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Víctor H BustamanteJosé L Puente
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Suzanne E OsborneBrian K Coombes
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeongjoon ChoiSangryeol Ryu
Apr 17, 2010·Science·Xiu-Jun YuDavid W Holden
Feb 20, 2007·Journal of Bacteriology·Umaporn SilphaduangBrian K Coombes
Mar 19, 2013·Journal of Bacteriology·Elena Cardenal-Muñoz, Francisco Ramos-Morales
May 26, 2009·Journal of Bacteriology·Omar H VandalSabine Ehrt
Mar 30, 2011·PloS One·Nat F BrownB Brett Finlay
Jan 9, 2014·PloS One·Leigh A KnodlerOlivia Steele-Mortimer
Nov 24, 2005·PLoS Pathogens·Nat F BrownB Brett Finlay
Apr 19, 2012·PLoS Pathogens·Douglas M HeithoffMichael J Mahan
Mar 4, 2011·Future Microbiology·Suzanne E Osborne, Brian K Coombes
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian K CoombesB Brett Finlay
May 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Charles AnsongLjiljana Pasa-Tolic

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.