PMID: 8598278Jan 1, 1996Paper

Expression and sequence analysis of a Treponema pallidum gene, tpn38(b), encoding an exported protein with homology to T. pallidum and Borrelia burgdorferi proteins

FEMS Microbiology Letters
L V StammJ G Frye

Abstract

An Escherichia coli clone containing recombinant plasmid C19 was identified from a Treponema pallidum genomic DNA library by in situ immunoassay. E. coli maxicells containing pC19 synthesized a treponemal protein doublet of 39.2 and 38.2 kDa, designated TpN38(b). Pulse-chase and protein processing studies showed that TpN38(b) is synthesized with a cleavable amino-terminal signal peptide. A 2.0-kb fragment of pC19 containing the tpn38(b) gene was subcloned and sequenced. The tpn38(b) gene is 1029 nucleotides long and encodes a protein of 343 amino acids with a calculated molecular mass of 37.9 kDa. The deduced amino acid sequence of TpN38(b) has homology with the T. pallidum TpN35 lipoprotein and the Borrelia burgdorferi BmpA, BmpB, BmpC, and BmpD proteins.

References

Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D B OliverG P Jarosik
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 15, 1985·Archives of Biochemistry and Biophysics·J B Stern, R C Jackson
Jun 1, 1982·Infection and Immunity·L V StammP J Bassford
Sep 1, 1994·Molecular Microbiology·J W Izard, D A Kendall
Jun 1, 1995·Molecular Microbiology·J D Radolf

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