Expression and subcellular localization of CDK2 and cdc2 kinases and their common partner cyclin A in thyroid epithelial cells: comparison of cyclic AMP-dependent and -independent cell cycles

Journal of Cellular Physiology
M BaptistPierre P Roger

Abstract

Dog thyroid epithelial cells in primary culture constitute a model of positive control of DNA synthesis initiation and G0-S prereplicative phase progression by cyclic AMP as a second messenger for TSH. In tis early steps, this mitogenic control is quite distinct from cyclic AMP-independent mitogenic cascades elicited by growth factors. We demonstrate here that TSH (cyclic AMP) and EGF+serum (cyclic AMP-independent) stimulations cooperate and finally converge on proteins that control the cell cycle machinery. This convergence included a common induction of the expression of cyclin A and p34cdc2, and to a lesser extent of p33/38cdk2, which was already expressed in quiescent thyroid cells, and common changes of cdc2 and CDK2 phosphorylations as evidenced by electrophoretic mobility shifts. Kinetic differences in these processes after stimulation by TSH or EGF+serum or by these factors in combination correlated with differences in cell cycle kinetics. Moreover, an immunofluorescence analysis of these proteins using the double labeling of PCNA as a marker of each cell cycle phase shows: (1) a previously undescribed nuclear translocation of CDK2 before S phase initiation; (2) a sudden increase of cdc2 nuclear immunoreactivity at G2/m...Continue Reading

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Citations

Sep 28, 2006·Journal of Cancer Research and Clinical Oncology·Chin-Ho ChoYong-Sung Juhnn
Sep 9, 2011·Molecular Biology of the Cell·Katia CoulonvalPierre P Roger
Jul 5, 2005·European Journal of Endocrinology·C CorrèzeM Pomérance
Jan 27, 2016·European Journal of Nuclear Medicine and Molecular Imaging·Daria Handkiewicz-JunakBarbara Jarzab
Oct 11, 2003·The Journal of Biological Chemistry·Katia CoulonvalPierre P Roger
Jul 16, 1999·Biochemical and Biophysical Research Communications·C DesdouetsJ Sobczak-Thepot
Dec 8, 2009·Molecular and Cellular Endocrinology·Pierre P RogerCarine Maenhaut

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