Expression and subcellular localization of the cyclin-dependent kinase inhibitor p27(Kip1) in epithelial ovarian cancer

Gynecologic Oncology
J HurteauM A Harrington

Abstract

To determine if p27(Kip1) expression was altered in epithelial ovarian cancers as compared to normal ovarian surface epithelial (NOSE) cells and to determine if subcellular localization of p27(Kip1) was an important feature. Thirteen tumor samples (1 Stage IC [early] and 12 Stage III/IV [advanced]) from patients with epithelial ovarian cancer and five NOSE samples were evaluated. Samples were surgically dissected to obtain an enriched population (90%) of cancer cells. The level of p27(Kip1) protein expression was determined by Western blot analysis. Actin was used as a loading control, and results were quantified by scanning densitometry using the ratio of the p27(Kip1) signal to the actin signal for comparison. To evaluate the subcellular localization of p27(Kip1), immunocytochemical staining was performed. Clinical pathological parameters were correlated to nuclear p27(Kip1) staining to establish if any association existed. When comparing the expression of p27(Kip1) between NOSE and ovarian cancer samples, only 2 of 13 ovarian cancer samples had altered p27(Kip1) expression. No correlation was found between the expression level of p27(Kip1) on Western blot and clinical pathological correlates. While no correlation between exp...Continue Reading

Citations

Dec 6, 2005·Journal of Cancer Research and Clinical Oncology·Annette Schmider-RossAngela Reles
Jul 23, 2015·Archives of Gynecology and Obstetrics·Mudan LuDaozhen Chen
Mar 7, 2008·Fertility and Sterility·Eduardo SchorVilmon de Freitas
Jul 16, 2013·Gynecologic Oncology·Heqiao DaiLinda H Malkas
Dec 3, 2009·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Timothy J DuncanLindy G Durrant

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