PMID: 8601692Apr 1, 1996Paper

Expression and tissue distribution of rat sulfated glycoprotein-1 (prosaposin)

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
C R MoralesS A Igdoura

Abstract

Sulfated glycoprotein-1 (SGP-1/prosaposin) exists as a sulfated secreted protein or as a lysosomal precursor of four smaller saposin molecules. The protein exhibits ubiquitous expression, evolutionary conservation, and diverse tissue inducibility. The lysosomal form of SGP-1 plays a role in the hydrolysis of glycolipids and sphingomyelin. The function of the secreted form of SGP-1 is still unclear. However, it could act as a glycolipid transfer protein, since several gangliosides (a series) were found to bind with high affinity to prosaposin. To identify cell types that produce SGP-1 mRNA, we constructed an SGP-1 cDNA and used for screening of different rat tissues by Northern blot analysis. To localize the translation product of SGP-1 transcripts, we immunostained the same tissues with an anti-SGP-1 antibody. The SGP-1 cDNA construct was generated by amplifying a rat testicular Zap cDNA library by PCR (polymerase chain reaction) with two synthetic oligonucleotide primers. A positive signal of 1.7 KB was isolated, subcloned into the pGEM-7Zf (+). Sequence analysis showed a near-identical nucleotide and amino acid similarity to a previous rat SGP-1 cDNA. The majority of the heterogeneites were conservative substitutions. Norther...Continue Reading

Citations

Feb 20, 2013·Cell and Tissue Research·Tetsuya ShimokawaSeiji Matsuda
Dec 19, 1998·Molecular Medicine Today·A E WillingP R Sanberg
Jan 23, 1999·The Biochemical Journal·L Madar-ShapiroM Horowitz
Mar 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuhiro Kohama
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Angel A Soler-GarcíaIra Pastan
Feb 14, 2012·Neuroscience Research·Omar Akil, Lawrence R Lustig
Aug 2, 2007·Traffic·Stéphane Lefrançois, Peter J McCormick
Aug 5, 2005·Genes, Chromosomes & Cancer·Shahriar KoochekpourOliver Sartor
Feb 1, 1997·Journal of Dairy Science·S PattonA Sano
May 2, 2003·Journal of Andrology·Louis Hermo, Sero Andonian
Feb 22, 2002·The Journal of Biological Chemistry·Stephane LefrancoisCarlos R Morales

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