Expression and tissue localization of collectin placenta 1 (CL-P1, SRCL) in human tissues

Molecular Immunology
Lana SelmanSoren Hansen

Abstract

Collectin placenta-1 (CL-P1), also known as scavenger receptor with C-type lectin (SRCL), is a type II membrane glycoprotein that shares structural features with both collectins and type A scavenger receptors. CL-P1 was originally cloned from the placenta and found to be associated with endothelial cells. It binds via its lectin domain to desialyated Lewis X containing glycoproteins and it is able to facilitate internalization of bound ligands. Via positively charged residues in the collagen-like region it binds to negatively charged components of microbial membranes. It has previously been proposed that CL-P1 plays a role in the host defense system and in the clearance of glycoproteins from the blood. With the aims of determining the detailed tissue expression of human CL-P1 we expressed CL-P1 recombinantly in both E. coli and CHO cells, and raised monoclonal antibodies against human CL-P1. Three monoclonal antibodies were characterized and used in immunohistochemical analyses of a panel of cryo- and formalin-fixed sections. We find that CL-P1 mainly associates with cytotrophoblasts and syncytiotrophoblasts of the placenta, alveolar macrophages and to a less degree with macrophage-like and stromal cells of the tonsils. By real...Continue Reading

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Citations

Dec 2, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gerald J Mizejewski
Nov 6, 2009·Antioxidants & Redox Signaling·Irena LevitanPapasani V Subbaiah
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May 10, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mercy R PrabhuDasJoseph El Khoury
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Feb 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mercy PrabhudasJoseph El Khoury
Nov 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maiken L HenriksenSoren Hansen
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Oct 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Soren HansenUffe Holmskov

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