PMID: 8949433Sep 1, 1996Paper

Expression and ultrastructural localization of HMB-45 antigen

The British Journal of Dermatology
A KikuchiT Nishikawa

Abstract

HMB-45 is a monoclonal antibody specific for melanoma cells and premature developing melanocytes. We examined the expression and specific subcellular binding sites of HMB-45 in various types of melanocytes including epidermal melanocytes from fetuses and infants with or without tyrosinase-negative oculocutaneous albinism (type IA), melanin-producing and non-producing melanoma cell lines (G361 and MeWo), and in vivo melanoma cells (melanotic and amelanotic malignant melanoma). Subcellular HMB-45 binding was examined by using post-embedding immunogold electron microscopy with rapid freezing and freeze substitution fixation methods without the use of chemical fixatives to preserve the intracytoplasmic delicate antigen property of HMB-45. HMB-45 antigen was detected not only in in vivo melanoma cells and normal fetal melanocytes, but also in melanocytes in the other conditions. Post-embedding immunogold electron microscopy revealed that HMB-45 antigen was exclusively localized to stages I and II melanosomes in the cytoplasm of neoplastic melanocytes, but was detected mainly on stages II and III melanosomes in the melanocytes from fetuses and infants. In tyrosinase-negative oculocutaneous albinism, only stages I and II melanosomes w...Continue Reading

References

Jun 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H ShimizuR A Eady
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Jan 1, 1995·Archives of Dermatological Research·A KikuchiT Nishikawa

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Citations

Nov 6, 2001·Molecular Biology of the Cell·J F BersonM S Marks
Oct 5, 2010·The American Journal of Dermatopathology·Asmaa Gaber AbdouNada Farag Elnaidany
Oct 23, 1997·Journal of Cutaneous Pathology·S CarrS Kossard
Dec 24, 1997·Journal of Cutaneous Pathology·V G Prieto, J M Woodruff
Jan 27, 2010·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Abdel Kader El TalDavid A Mehregan
Jul 18, 2012·Dermatologic Clinics·Andrea M HuiDaniel M Siegel
Aug 15, 2009·European Journal of Cell Biology·Regina Kuliawat, Laura Santambrogio
May 29, 2012·Acta Ophthalmologica·Nils EideØystein Fodstad
Apr 21, 2004·Journal of the American Academy of Dermatology·Hatem M El-GamalMartin C Mihm
Sep 1, 2011·Toxicological Research·So-Young Choi, Young-Chul Kim
Mar 31, 2015·Journal of Dermatological Science·Motoki NakamuraAkimichi Morita
May 29, 2000·The Journal of Dermatology·Z H JinS Kinebuchi
Oct 21, 1999·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·A Thybusch-BernhardtH Juhl
Apr 20, 2001·Journal of the American Academy of Dermatology·G M MenakerR L Moy
Feb 25, 2003·International Journal of Surgical Pathology·Hadi Yaziji, Allen M Gown
Sep 2, 2003·International Journal of Cancer. Journal International Du Cancer·Xin GuanShun'ichiro Taniguchi
Jan 8, 2003·Pathology Oncology Research : POR·Samina NooraliShahid Pervez
Apr 21, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S RegauerA Beham
Aug 3, 2011·Expert Review of Anticancer Therapy·Jeremy R EtzkornL Frank Glass

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