Abstract
Brucella abortus is a bacterium of brucellosis causing abortion in cattle. The diagnosis of bovine brucellosis mainly relies on serologic tests using smooth lipopolysaccharide (S-LPS) from B. abortus. However, the usefulness of this method is limited by false-positive reactions due to cross-reaction with other Gram-negative bacteria. In the present study, the eryC gene encoding B. abortus d-erythrulose 1-phosphate dehydrogenase, which is involved in the erythritol metabolism in virulent B. abortus strain but is absent from a B. abortus vaccine strain (S19), was cloned. Recombinant EryC was expressed and purified for the evaluation as a diagnostic reagent for bovine brucellosis. Other B. abortus proteins, Omp16, PP26, and CP39 were also purified and their seroreactivities were compared. Recombinant EryC, Omp16, PP26, and PP39 were all reactive to B. abortus-positive serum. The specificity of recombinant Omp16, PP26, CP39, and EryC, were shown to be approximately 98%, whereas that of B. abortus whole cell lysates was shown to be 95%. The sensitivity of Omp16, PP26, CP39, and EryC were 10%, 51%, 64%, and 43%, respectively, whereas that of B. abortus whole cell lysates was 53%. These results suggested that B. abortus EryC would be ...Continue Reading
References
Feb 1, 1975·Journal of Bacteriology·J F Sperry, D C Robertson
Oct 1, 1975·Journal of Bacteriology·J F Sperry, D C Robertson
Dec 1, 1992·Journal of Clinical Microbiology·A CloeckaertJ N Limet
Mar 11, 1992·Clinical and Experimental Immunology·J Y ParkJ D Kim
Dec 1, 1990·Infection and Immunity·A CloeckaertJ N Limet
Jan 1, 1990·Experimental Physiology·M Lakie, L G Robson
May 1, 1988·Journal of Clinical Microbiology·J A DurhamA W Confer
Oct 1, 1987·Journal of Clinical Microbiology·N P MoyerW J Hausler
Nov 1, 1970·Analytical Biochemistry·A L Koch
Mar 1, 1967·Journal of Bacteriology·M E Meyer
Jan 28, 1983·Journal of Immunological Methods·M D RichardsonP A Llewellyn
May 1, 1995·Clinical and Diagnostic Laboratory Immunology·F HemmenE Saman
Sep 1, 1994·Infection and Immunity·A TiborJ J Letesson
Apr 1, 1993·Comparative Immunology, Microbiology and Infectious Diseases·L E Samartino, F M Enright
Aug 1, 1993·Journal of Clinical Microbiology·F A GoldbaumC A Fossati
Jan 1, 1996·Infection and Immunity·A TiborJ J Letesson
Mar 1, 1996·Infection and Immunity·M G Stevens, S C Olsen
Jul 1, 1996·Infection and Immunity·L E LindlerR L Warren
Jan 1, 1996·Veterinary Microbiology·V WeynantsJ J Letesson
Jan 1, 1996·Journal of Clinical Microbiology·O L RossettiS L Cravero
Oct 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S C OlsenM G Stevens
Feb 1, 1997·Infection and Immunity·P A DenoelJ J Letesson
May 1, 1997·Clinical and Diagnostic Laboratory Immunology·J VelascoI Moriyón
May 22, 1998·Veterinary Microbiology·R KittelbergerJ J Letesson
Jun 10, 1998·Journal of Clinical Microbiology·A B MikolonA J Edmondson
Jul 3, 1999·Clinical and Diagnostic Laboratory Immunology·A CloeckaertM S Zygmunt
Mar 9, 2000·Microbiology·F J SangariJ M García-Lobo
Apr 20, 2002·Current Opinion in Infectious Diseases·Kimberly A Yeung-YueStephen K Tyring
Jul 11, 2003·Clinical and Diagnostic Laboratory Immunology·Patricia Seco-MediavillaNieves Vizcaíno
Jan 6, 1962·Nature·H SMITHK WITT
May 12, 2004·Veterinary Microbiology·K NielsenP Nicoletti
Dec 21, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Al DahoukH Neubauer
Jun 2, 2006·Proteomics·Joseph P ConnollyVito G DelVecchio
Jul 7, 2007·Journal of Immunoassay & Immunochemistry·K NielsenG Halbert
Aug 10, 2007·Biotechnology and Applied Biochemistry·Sanjay KumarHarsh Vardhan Batra
Aug 30, 2007·Hybridoma·Sanjay KumarHarsh Vardhan Batra