Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae

Yeast
M A van den Berg, H Y Steensma

Abstract

We employed two genes in constructing yeast expression cassettes for dominant, selectable markers. The Saccharomyces cerevisiae gene SFA1, encoding formaldehyde dehydrogenase, was placed under the control of the GPD1 promoter and CYC1 terminator. The Moraxella sp. strain B gene dehH1, encoding fluoroacetate dehalogenase, was placed under the control of both the GPD1 and CYC1 promoters. With these constructs it was possible to select directly for yeast strains resistant to either formaldehyde or fluoroacetate. Both selective agents are completely metabolized and inexpensive, making them very useful in the pursuit of yeast gene functions and for industrial applications. An additional advantage of the formaldehyde dehydrogenase marker is that it is an S. cerevisiae gene, thus allowing 'all yeast' constructs.

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Citations

Apr 27, 2002·Applied and Environmental Microbiology·Jack T Pronk
Apr 2, 2008·Applied and Environmental Microbiology·Richard J S BaerendsJack T Pronk
Nov 28, 2013·BMC Genomics·Nikolai V RavinKonstantin G Skryabin
Oct 20, 2005·Journal of Bioscience and Bioengineering·Rinji Akada
May 9, 2006·Molecular Microbiology·Mikael Molin, Anders Blomberg
Dec 21, 2012·FEMS Yeast Research·Daniel Solis-EscalantePascale Daran-Lapujade
Mar 28, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Tatsuo Kurihara, Nobuyoshi Esaki
Feb 13, 2001·Yeast·M Mollapour, P Piper
May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thiau-Fu AngThean Chor Leow
Dec 22, 2009·Applied Biochemistry and Biotechnology·Hong ZhuShilong Wang
May 21, 2009·Applied Microbiology and Biotechnology·Shohei KanekoAkihiko Kondo
Jun 7, 2012·G3 : Genes - Genomes - Genetics·Mark K Chee, Steven B Haase
Nov 28, 2012·World Journal of Microbiology & Biotechnology·Changying Guo, Ning Jiang
Feb 11, 2014·Current Protocols in Molecular Biology·Zarmik Moqtaderi, Joseph V Geisberg
Nov 12, 2015·Biochemistry. Biokhimii︠a︡·E V TereshinaS I Ivanenko

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