Expression cloning and biochemical characterizations of recombinant cyclophilin proteins from Schistosoma mansoni

Protein Expression and Purification
F BugliM Q Klinkert

Abstract

Recombinant Schistosoma mansoni cyclophilin proteins of the A and the B subtypes (SmCYP A and B) were expressed in bacterial cells as histidine- and maltose-binding fusion proteins and also as nonfused proteins. In addition, S. mansoni CYPs were produced in Sf9 insect cells in their natural forms. Purified recombinant SmCYP B was found to possess a peptidyl-prolyl cis-trans isomerase (PPIase) activity, with a kcat/Km value of 8.2 x 10(5) M-1 s-1. The SmCYP B isoform is approximately two to three times more active than SmCYP A. SmCYP B-specific RNA appears to be more abundant in adult schistosomes than SmCYP A RNA in Northern blots. These results support the conclusion that SmCYP B represents the major schistosomal CYP. The PPIase-associated activity of both CYPs was inhibitable by the immunosuppressive drug cyclosporin A (CsA). We attempt to explain differences in PPIase activities and in CsA inhibition by examining models of the two CYPs complexed to CsA.

References

May 15, 1992·European Journal of Biochemistry·T ThalhammerR E Handschumacher
Jul 1, 1991·Molecular and Cellular Biology·K W HaselJ G Sutcliffe
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E R PriceC T Walsh
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J LiuC T Walsh
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S SchneuwlyW L Pak
Nov 2, 1984·Science·R E HandschumacherD W Speicher
Jul 1, 1981·Agents and Actions·E BuedingY N Cha
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·V MikolM D Walkinshaw
Oct 30, 1996·Molecular and Biochemical Parasitology·M Q KlinkertD Cioli

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Citations

May 15, 2018·Frontiers in Medicine·Ruben FernandesMónica C Botelho
Nov 17, 2020·Frontiers in Plant Science·Harpreet SinghPrabhjeet Singh
May 26, 1999·Molecular and Biochemical Parasitology·A KhattabM Q Klinkert

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