PMID: 9177479May 2, 1997Paper

Expression cloning and intracellular localization of a human ZF5 homologue

Biochimica Et Biophysica Acta
K SugiuraM Hagiwara

Abstract

We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

Citations

Jan 7, 2004·Biochemical and Biophysical Research Communications·Ki-Hwan LeeYong-Sung Lee
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·T CathomenM D Weitzman
Jun 30, 2000·Biochemical and Biophysical Research Communications·P M AllielJ P Périn
Jan 5, 2005·Biochemical and Biophysical Research Communications·Jun WangNobuyoshi Shimizu
Dec 29, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Ki-Hwan Lee, Yong-Sung Lee
Dec 31, 1997·FEBS Letters·Y NagaiM Hagiwara
Nov 25, 2005·Journal of Cell Science·Tae-Aug KimShalom Avraham

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