PMID: 9440522Jan 24, 1998Paper

Expression cloning of a Pseudomonas gene encoding a hydroxydecanoyl-acyl carrier protein-dependent UDP-GlcNAc acyltransferase

Journal of Bacteriology
G D DotsonC R Raetz

Abstract

UDP-N-acetylglucosamine-3-O-acyltransferase (UDP-GlcNAc acyltransferase) catalyzes the first step of lipid A biosynthesis (M. S. Anderson and C. R. H. Raetz, J. Biol. Chem. 262:5159-5169, 1987). We here report the isolation of the lpxA gene of Pseudomonas aeruginosa from a library of Pseudomonas strain PAO1 expressed in Escherichia coli LE392 (J. Lightfoot and J. S. Lam, J. Bacteriol. 173:5624-5630, 1991). Pseudomonas lpxA encodes a 10-carbon-specific UDP-GlcNAc acyltransferase, whereas the E. coli transferase is selective for a 14-carbon acyl chain. Recombinant cosmid 1137 enabled production of a 3-hydroxydecanoyl-specific UDP-GlcNAc acyltransferase in E. coli. It was identified by assaying lysozyme-EDTA lysates of individual members of the library with 3-hydroxydecanoyl-acyl carrier protein (ACP) as the substrate. Cosmid 1137 contained a 20-kb insert of P. aeruginosa DNA. The lpxA gene region was localized to a 1.3-kb SalI-PstI fragment. Sequencing revealed that it contains one complete open reading frame (777 bp) encoding a new lpxA homolog. The predicted Pseudomonas LpxA is 258 amino acids long and contains 21 complete hexapeptide repeating units, spaced in approximately the same manner as the 24 repeats of E. coli LpxA. Th...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Feb 1, 1992·Journal of Bacteriology·A P MoranT U Kosunen
Dec 1, 1987·Journal of Bacteriology·D N CrowellC R Raetz
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·L YuanD J Hawkins
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Nov 8, 1996·Science·H R OnishiC R Raetz
Jan 21, 1997·Biochemistry·T W BeamanS L Roderick

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Citations

Aug 28, 2009·Innate Immunity·Jerry D KingJoseph S Lam
Mar 4, 2014·Annual Review of Biochemistry·Chris Whitfield, M Stephen Trent
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Allison H Williams, Christian R H Raetz
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Stephen Trent
Nov 5, 2015·Biomolecules & Therapeutics·Sang Hoon Joo
Mar 10, 2019·Scientific Reports·Manchuta DangkulwanichAllison H Williams
Nov 26, 1998·The Journal of Biological Chemistry·T J WyckoffC R Raetz
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Feb 9, 2008·Molecular Plant-microbe Interactions : MPMI·Karyn L NewmanSteven E Lindow
May 24, 2013·Microbiology and Molecular Biology Reviews : MMBR·Annika Röttig, Alexander Steinbüchel
Apr 22, 2008·Biochemistry·Craig M Bartling, Christian R H Raetz
Mar 5, 1999·Biochimica Et Biophysica Acta·T A GarrettC R Raetz
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·P StanleyC Hughes
Dec 6, 2005·Protein Expression and Purification·Gregory A ZornetzerBrian G Fox
Jun 12, 2019·Journal of the American Chemical Society·Karthik SankaranarayananChaitan Khosla

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