Expression, cross-linking, and characterization of recombinant chitin binding resilin

Biomacromolecules
Guokui QinDavid L Kaplan

Abstract

Resilin is a polymeric rubber-like protein secreted by insects to specialized cuticle regions, in areas where high resilience and low stiffness are required. Resilin binds to the cuticle polysaccharide chitin via a chitin binding domain and is further polymerized through oxidation of the tyrosine residues resulting in the formation of dityrosine bridges and assembly of a high-performance protein--carbohydrate composite material. We describe the mechanical, structural and biochemical function of chitin binding recombinant Drosophila melanogaster resilin. Various resilin constructs were cloned including the full length gene enabling Ni-NTA purification, as well as heat and salt precipitation for rapid and efficient purification. The binding isotherms and constants (K(d), B(max)) of resilin to chitin via its chitin binding domain were determined and displayed high affinity to chitin, implying its important role in the assembly of the resilin-chitin composite. The structural and elastic properties were investigated using Fourier transform infrared spectroscopy, circular dichroism, and atomic force microscopy with peroxidase cross-linked solid resilin materials. Generally, little structural organization was found by these biophysica...Continue Reading

References

May 1, 1990·Biotechnology Progress·J CappelloF Ferrari
Dec 1, 1989·Journal of Biomedical Materials Research·F LefebvreM Rabaud
Jun 1, 1966·Journal of Insect Physiology·G C Coles
Jul 11, 1998·Journal of Biomaterials Science. Polymer Edition·R Yoda
Sep 13, 2000·Proceedings. Biological Sciences·F HaasR Blickhan
Aug 3, 2001·Insect Biochemistry and Molecular Biology·D H Ardell, S O Andersen
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John GoslineKen Savage
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Arthur S Tatham, Peter R Shewry
Jul 20, 2002·Science·Joe Alper
Oct 18, 2002·Advanced Drug Delivery Reviews·Zaki MegeedHamidreza Ghandehari
Oct 9, 1964·Biochimica Et Biophysica Acta·S O ANDERSEN
Oct 14, 2005·Nature·Christopher M ElvinNicholas E Dixon
Jan 16, 2007·Protein Engineering, Design & Selection : PEDS·Russell E LyonsChristopher M Elvin
Apr 5, 2007·Nature Protocols·Norma J Greenfield
Jul 1, 2008·Biophysical Journal·Kate M NairnChristopher M Elvin
Jan 1, 2009·Soft Matter·Manoj B CharatiKristi L Kiick

❮ Previous
Next ❯

Citations

May 11, 2011·Biomacromolecules·Linqing LiKristi L Kiick
Oct 24, 2013·Biomacromolecules·Renay S-C SuJulie C Liu
Feb 7, 2013·Journal of the American Chemical Society·Zhongyuan SunDavid L Kaplan
Aug 16, 2012·Nature Communications·Guokui QinDavid L Kaplan
Jul 9, 2013·Acta Biomaterialia·Renay S-C SuJulie C Liu
Sep 22, 2012·Nanomedicine·Shaul LapidotOded Shoseyov
Jun 4, 2011·Journal of Polymer Science. Part A, Polymer Chemistry·Linqing LiKristi L Kiick
Feb 7, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Md Shahriar K KhandakerDavid R Bevan
Jan 18, 2016·Micron : the International Research and Review Journal for Microscopy·Rakkiyappan ChandranDennis LaJeunesse
Feb 13, 2016·Current Opinion in Biotechnology·Natalia E DomeradzkaRenko de Vries
Jul 15, 2015·Advanced Healthcare Materials·Alessandra GirottiJosé Carlos Rodríguez-Cabello
Oct 4, 2011·Biomaterials·Guokui QinDavid L Kaplan
Sep 1, 2011·Insect Biochemistry and Molecular Biology·Russell E LyonsChristopher M Elvin
Aug 3, 2010·Biomaterials·Xiao HuDavid L Kaplan
Aug 21, 2013·Macromolecules·Christopher L McGannKristi L Kiick
Jan 26, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Darren C C WongDavid J Merritt
Jun 26, 2012·Advanced Materials·Rebecca L DiMarco, Sarah C Heilshorn
Aug 6, 2014·Advanced Functional Materials·Xiao HuDavid L Kaplan
Nov 14, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Malav S Desai, Seung-Wuk Lee
Nov 6, 2013·Carbohydrate Polymers·Guokui QinZhongyuan Sun David L Kaplan
Dec 17, 2011·Protein Expression and Purification·Julie N RennerJulie C Liu
Feb 2, 2012·European Journal of Cell Biology·Khaleelulla Saheb ShaikBernard Moussian
Mar 31, 2015·Nanomedicine·Yinan LinDavid L Kaplan
May 20, 2014·Biomaterials·Raquel SilvaAldo R Boccaccini
Oct 11, 2016·Current Opinion in Insect Science·Mi Young NohYasuyuki Arakane
Sep 3, 2013·ACS Macro Letters·Linqing Li, Kristi L Kiick
Nov 4, 2016·Arthropod Structure & Development·Laura Vannini, Judith H Willis
Jun 2, 2017·Biomacromolecules·Isaac WeitzhandlerAshutosh Chilkoti
Jan 21, 2012·Biomedical Materials·Jerome A Werkmeister, John A M Ramshaw
Sep 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rajkamal BaluNamita R Choudhury
Jul 26, 2017·Biopolymers·Rachael N ParkerTijana Z Grove
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ke ZhengDavid L Kaplan
Oct 7, 2015·Advanced Functional Materials·David W HowellSarah E Bondos
Sep 2, 2020·Advanced Drug Delivery Reviews·Anastasia VarankoAshutosh Chilkoti
Nov 5, 2020·International Journal of Biological Macromolecules·Yu LiArtur Cavaco-Paulo
Jan 22, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wonkyung AhnEun Jung Lee
Jan 19, 2021·Biomacromolecules·Sheng-Chen HuangZhi-Gang Qian
Dec 16, 2020·BMC Biology·Steven LerchBernard Moussian
Dec 16, 2020·Polymers·Xuan MuDavid L Kaplan
Aug 30, 2017·International Journal of Biological Macromolecules·Shalini V GohilLakshmi S Nair

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.