Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide

Veterinary Immunology and Immunopathology
Muhammad Jasim UddinMehmet Ulas Cinar

Abstract

The Toll-like receptor (TLR)4 is critical for the recognition of Gram-negative bacterial lipopolysaccharide (LPS) but in porcine peripheral blood mononuclear cells (PBMCs) it may cooperate with other TLRs and lead to the production of inflammatory cytokines. Therefore, we analyzed TLR1-10 mRNA expression in porcine PBMCs stimulated with LPS over time (1-48 h) by using quantitative real-time PCR and cytokine proteins level by ELISA in culture supernatant. TLR1-10 mRNA was detectable in porcine PBMCs. When compared with the control (non-stimulated), TLR1 mRNA were increased (p<0.05) at 3 h after challenge with 1 μg/ml LPS, whereas TLR1 and TLR2 mRNA were increased (p<0.01) at 6 h after challenge with 10 μg/ml LPS. TLR4 increased (p<0.001) at 3h after challenge with LPS and remained constant. TLR5 and TLR6 mRNA increased (p<0.05) at 9 h and 1 h after of LPS stimulation, respectively. The mRNA of CD14 and MD2 were increased (p<0.001) at 1h after LPS stimulation. Additionally, at most of the time analyzed, the mRNA expression increased with the dose of LPS. The LPS concentration had influence (p<0.05) on all the TLRs expression except TLR10; whereas time had effect (p<0.05) on all TLRs expression except TLR2, 3, 6 and 10. When compa...Continue Reading

References

May 18, 2001·Veterinary Immunology and Immunopathology·H YancyM J Myers
Jul 31, 2001·The Journal of Biological Chemistry·F Re, J L Strominger
Jul 23, 2002·Trends in Immunology·Michael Rehli
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Jan 22, 2005·Veterinary Immunology and Immunopathology·Muhammad IqbalAdrian L Smith
Apr 15, 2005·Molecular Immunology·I KokkinopoulosM A Ritter
Jun 21, 2005·Veterinary Immunology and Immunopathology·Glicerio IgnacioGregg A Dean
Aug 13, 2005·Veterinary Immunology and Immunopathology·Moira Menzies, Aaron Ingham
Jul 3, 2007·Veterinary Immunology and Immunopathology·King S NalubambaJohn Hopkins
Jul 4, 2007·Proceedings of the American Thoracic Society·Arthur M Krieg
Apr 29, 2008·Molecular Immunology·Jianzhong ZhuGeorge K Mutwiri
Jul 2, 2008·Developmental and Comparative Immunology·Hirohide Uenishi, Hiroki Shinkai
Jul 16, 2008·Current Opinion in Immunology·Sachiko Akashi-Takamura, Kensuke Miyake
Jul 30, 2009·The Journal of Biological Chemistry·Sumanta MukherjeeZhixing K Pan
Sep 15, 2009·Veterinary Immunology and Immunopathology·K G TirumurugaanV Ramaswamy
Oct 21, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ebbe Billmann ThorgersenTom Eirik Mollnes
Aug 20, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N E GomesR Salomão
Nov 16, 2010·Veterinary Immunology and Immunopathology·Nanna S SorensenPeter M H Heegaard
Jan 15, 2011·Journal of Leukocyte Biology·Lynsey FairbairnDavid A Hume
Mar 1, 2012·Veterinary Immunology and Immunopathology·Mohammad Ariful IslamKarl Schellander

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Citations

Dec 24, 2015·Frontiers in Veterinary Science·Muhammad J UddinHelle Bielefeldt-Ohmann
May 16, 2015·The Journal of Infectious Diseases·Hiroyuki NagashimaYoshio Yamaoka
Jul 22, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Yijie GuoEmiko Isogai
Apr 9, 2014·Developmental and Comparative Immunology·K H MairF Meurens
Nov 16, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Martine Schroyen, Christopher K Tuggle
May 27, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yulan LiuGuolong Zhang
Mar 21, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joonsup LeePeter A Lay
Jan 5, 2021·Tropical Animal Health and Production·Esma Gamze Aksel, Bilal Akyüz

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