PMID: 9181118Feb 1, 1997Paper

Expression from cardiomyocyte-specific promoter after adenovirus-mediated gene transfer in vitro and in vivo

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
F GriscelliT Ragot

Abstract

Adenoviruses are very attractive vectors for gene transfer into the cardiac muscle; however, their promiscuous tissue tropism, leading to an ectopic expression of the transgene, is a considerable practical limitation. To restrict expression of a reporter gene in cultured cardiomyocytes and in the heart of the rat, we have constructed a recombinant adenovirus (Ad-MLC2 beta gal) containing the beta-galactosidase gene under the control of the rat ventricle-specific cardiac myosin light chain 2 (MLC-2v) promoter. We show in this work that the MLC-2v promoter inside the adenoviral genome retains its cardiac specificity in vitro in cultured cardiomyocytes as well as in vivo in the animal heart. Northern blot studies after Ad-MLC2 beta gal infection show significant transcription only in cells derived from the cardiac muscle and not from the skeletal muscle. Quantitative analysis of the beta-galactosidase activity in a number of cell lines also confirms this result. The level of beta-galactosidase expression in rat neonatal cardiomyocytes infected with Ad-MLC2 beta gal is 8% of that found when primary cells are infected with Ad-RSV beta gal (containing a beta-galactosidase gene under the control of the Rous sarcoma virus promoter). Th...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·L D Stratford-PerricaudetP Briand
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L BessereauJ P Changeux
Sep 1, 1993·Neuromuscular Disorders : NMD·C Rücker-MartinJ J Mercadier
Aug 1, 1996·The Annals of Thoracic Surgery·C J MagovernT K Rosengart
Jan 20, 1996·Human Gene Therapy·M HashimotoK Mikoshiba

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Citations

Nov 9, 2005·Blood Reviews·Paul H Lerou, George Q Daley
Sep 28, 1999·Clinical and Experimental Pharmacology & Physiology·M Y AlexanderH M Prentice
Mar 7, 2014·American Journal of Physical Anthropology·Aline Thomas

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