PMID: 8961556Dec 1, 1996Paper

Expression in a wine yeast strain of the Aspergillus niger abfB gene

FEMS Microbiology Letters
P Sánchez-TorresD Ramón

Abstract

A recombinant wine yeast strain has been constructed expressing the gene coding for alpha-L-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous alpha-L-arabinofuranosidase B shows similar physico-chemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The alpha-L-arabinofuranosidase B protein is detected throughout the fermentation.

References

Dec 1, 1992·Applied Microbiology and Biotechnology·R GonzálezJ A Pérez-González
Mar 1, 1995·FEMS Microbiology Letters·L González-CandelasD Ramon
Oct 1, 1993·FEMS Microbiology Letters·D RamónJ Visser
Sep 1, 1992·Applied and Environmental Microbiology·A QuerolD Ramón

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Citations

Nov 11, 2010·FEMS Yeast Research·Anscha J J ZietsmanPierre van Rensburg
Dec 19, 2000·Journal of Applied Microbiology·M VilanovaC Sieiro
Dec 31, 2005·Journal of Industrial Microbiology & Biotechnology·Mondher Th Numan, Narayan B Bhosle
Feb 5, 2008·Applied and Environmental Microbiology·Manuel Ramírez, Jesús Ambrona
Apr 28, 2005·Applied Microbiology and Biotechnology·Dorit Schuller, Margarida Casal
Nov 23, 2006·Biotechnology Letters·Eduardo CebolleroRamon Gonzalez
Feb 7, 2001·Journal of Agricultural and Food Chemistry·P Sánchez-TorresD Ramón

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