PMID: 2123631Oct 1, 1990Paper

Expression in Escherichia coli and purification of human immunodeficiency virus type 1 capsid protein (p24)

AIDS Research and Human Retroviruses
L S EhrlichC A Carter

Abstract

Capsid protein (p24;CA) of human immunodeficiency virus type 1 (HIV-1) was synthesized in Escherichia coli strain BL21 (DE3) using a plasmid encoding a truncated HIV-1 gag/pol gene. The plasmid, which contained a mutation in the frameshift region, expressed viral proteinase (PR), a pol gene product, in the gag reading frame, resulting in efficient processing of mature CA and other gag-related products. The expressed CA is soluble, recognized by monoclonal antibodies directed against HIV CA and has an N-terminal sequence identical to that of CA purified from HIV. Purification was done under mild conditions where coexpressed HIV PR retained enzymatic activity. Milligram quantities of 90% pure CA protein were obtained after chromatography on DEAE cellulose followed by facilitated aggregation of the CA in the unbound fraction. The precipitated CA was readily dissolved in low ionic strength aqueous buffer. Gel exclusion chromatography results indicated that, in solution, CA existed in oligomeric form.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W A HaseltineW Gilbert
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J ShineH M Goodman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H G KräusslichC A Carter
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T D MeekC Debouck
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Jan 1, 1988·Annual Review of Biochemistry·H G Kräusslich, E Wimmer
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N E KohlI S Sigal
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C DebouckM Rosenberg
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt

❮ Previous
Next ❯

Citations

May 1, 1995·Journal of Virological Methods·S R Kelsall, J K Kulski
Nov 1, 1993·Journal of Biotechnology·I MarczinovitsJ Molnár
Sep 1, 1996·Nature Structural Biology·C MomanyM G Rossmann
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J ProngayJ McClure
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K PartinC Carter
May 6, 2011·Journal of Virology·G N MedinaC A Carter
Mar 9, 2013·PloS One·Magdeleine HungRoman Sakowicz
Aug 31, 2011·Journal of Molecular Biology·Lorna S EhrlichCarol A Carter
Jul 19, 2011·Journal of Molecular Biology·Lorna S EhrlichCarol A Carter
Jun 6, 1998·Journal of Molecular Biology·S ScarlataC A Carter
Aug 1, 1992·Journal of Virology·J LubanS P Goff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Matthew Scott Lalonde, Wesley I Sundquist
© 2021 Meta ULC. All rights reserved