Expression in mammalian cell cultures reveals interdependent, but distinct, functions for Star and Rhomboid proteins in the processing of the Drosophila transforming-growth-factor-alpha homologue Spitz

The Biochemical Journal
John C PascallKenneth D Brown

Abstract

We report here distinct interdependent functions for two proteins, Star and Rhomboid, that are key determinants of the epidermal-growth-factor (EGF)-receptor signalling pathway in Drosophila. When we expressed the Drosophila EGF-receptor ligand Spitz in mammalian cells, the protein failed to traffic to the plasma membrane, as assessed by either cell-surface protein biotinylation or immunocytochemical staining. However, when we co-expressed Star with Spitz, trafficking of Spitz to the cell surface could be demonstrated. Only when we co-expressed Spitz, Star and Rhomboid could the release of soluble Spitz protein into the medium be shown. Taken together, our results indicate that Star is required for the intracellular trafficking of Spitz, and that Rhomboid is essential for the release of soluble Spitz protein from cells.

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Citations

Mar 30, 2004·Biochemical and Biophysical Research Communications·John C Pascall, Kenneth D Brown
Jul 9, 2008·Annual Review of Genetics·Matthew Freeman
Oct 4, 2005·Developmental Cell·Angelike Stathopoulos, Michael Levine
Mar 16, 2005·Developmental Biology·Amy Ralston, Seth S Blair
Nov 22, 2008·Seminars in Cell & Developmental Biology·Matthew Freeman
Nov 18, 2008·Experimental Cell Research·Carl P BlobelMatthew Freeman
Mar 12, 2004·Developmental Biology·Jennie B DormanCeleste A Berg
Sep 15, 2007·Molecular Cancer Research : MCR·Martín C AbbaC Marcelo Aldaz
Dec 27, 2007·Molecular Cell·Marius K Lemberg, Matthew Freeman

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