β-Nerve growth factor (β-NGF) is a seminal plasma element, responsible for inducing ovulation in camelids. The main organ of β-NGF production remains nondescript. The aims of this study were to (a) characterize gene expression and protein localization of β-NGF and its main receptor tyrosine kinase receptor A (TrKA) in the llama male reproductive tract, and (b) determine whether the seminal β-NGF interacts with ejaculated sperm by localizing β-NGF and TrKA in epididymal, ejaculated, and acrosome-reacted (AR) sperms and, additionally, by identifying β-NGF presence in sperm-adsorbed proteins (SAP). Both β-NGF and TrkA transcripts are widely expressed along the male reproductive tract, with a higher expression level of β-NGF at prostate (p < 0.05). β-NGF immunolabeling was only positive for prostate, whereas TrKA label was present in epithelial and muscular cells of testis, prostate, bulbourethral glands, and epididymis. Using an immunofluorescent technique, β-NGF was colocalized with TrKA in the middle piece of ejaculated and AR sperm. However, only TrKA was observed in epididymal sperm indicating that β-NGF could have a seminal origin. This was also confirmed by the identification of four β-NGF isoforms in SAP. This study extends...Continue Reading
Expression of beta-nerve growth factor and its receptor in rat seminiferous epithelium: specific function at the onset of meiosis
Essential problems in quantification of proteins following colloidal staining with coomassie brilliant blue dyes in polyacrylamide gels, and their solution
Expression of the beta-nerve growth factor gene in male sex organs of the mouse, rat, and guinea pig
Immunohistochemical localization of nerve growth factor and epidermal growth factor in guinea pig prostate gland
Expression of nerve growth factor and nerve growth factor receptor tyrosine kinase Trk in activated CD4-positive T-cell clones
Bovine seminal plasma aSFP: localization of disulfide bridges and detection of three different isoelectric forms
Expression of mRNAs for neurotrophic factors (NGF, BDNF, NT-3, and GDNF) and their receptors (p75NGFR, trkA, trkB, and trkC) in the adult human peripheral nervous system and nonneural tissues
Expression of nerve growth factor and neurotrophin receptors in testicular cells suggest novel roles for neurotrophins outside the nervous system
Characterization of nerve growth factor precursor protein expression by human prostate stromal cells: a role in selective neurotrophin stimulation of prostate epithelial cell growth
Spatiotemporal patterns of expression of neurotrophins and neurotrophin receptors in mice suggest functional roles in testicular and epididymal morphogenesis
Expression of nerve growth factor (NGF), and its receptors TrkA and p75 in the reproductive organs of the adult male rats
Collection method, season and individual variation on seminal characteristics in the llama (Lama glama)
Cryopreservation of epididymal alpaca (Vicugna pacos) sperm: a comparison of citrate-, Tris- and lactose-based diluents and pellets and straws
Absence of nerve growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men
Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function
Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas
Effects of castration on the expression of the NGF and TrkA in the vas deferens and accessory male genital glands of the rat.
β-Nerve growth factor is a major component of alpaca seminal plasma and induces ovulation in female alpacas
Immunolocalization of NGF and its receptors in ovarian surface epithelium of the wild ground squirrel during the breeding and nonbreeding seasons
Effect of cryoprotectant and equilibration temperature on cryopreservation of Lama glama spermatozoa
Discovery, primary, and crystal structures and capacitation-related properties of a prostate-derived heparin-binding protein WGA16 from boar sperm.
Gene Expression and Localization of NGF and Its Cognate Receptors NTRK1 and NGFR in the Sex Organs of Male Rabbits
Seminal Plasma Induces Ovulation in Llamas in the Absence of a Copulatory Stimulus: Role of Nerve Growth Factor as an Ovulation-Inducing Factor
Source and localization of ovulation-inducing factor/nerve growth factor in male reproductive tissues among mammalian species
Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits
Effect of mating on mRNA and protein expression of beta nerve growth factor and its receptor, TrKA, in the oviduct of llama (Lama glama).
New Insights Into the Role of β-NGF/TrKA System in the Endometrium of Alpacas During Early Pregnancy.
Cell Signaling by Tyrosine Kinases
Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.