Expression of 11 beta-hydroxysteroid dehydrogenase during decidualization of human endometrial stromal cells

Endocrinology
F ArcuriFrederick Schatz

Abstract

This study evaluated the expression of the corticosteroid-metabolizing enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) during in vitro decidualization of human endometrial stromal cells. The cultured stromal cells displayed both NADP(+)-dependent (type 1) and NAD(+)-dependent (type 2) 11 beta HSD activities under basal conditions. Although the cells did not respond to estradiol (E2) added alone, catalytic levels of both isoforms were enhanced by medroxyprogesterone acetate (MPA) and further enhanced by E2 plus MPA. Type I messenger RNA (mRNA) was undetected by Northern analysis of total RNA, but was evident as a 1.5-kilobase band in polyadenylated selected RNA from E2- plus MPA-treated cultures. Use of RT-PCR to augment the sensitivity of mRNA detection revealed the presence of type I mRNA as a faint band in the MPA-treated cultures and as an intense band in the E2- plus MPA-treated cultures. Thus, type I mRNA is present as a low abundance message in the cultured stromal cells whose steady state levels parallel progestin-enhanced enzyme activity. As the expression of several progestin-regulated decidualization markers is also augmented by E2, the results of the present study reveal a correlation between enhanced 11 be...Continue Reading

Citations

Sep 2, 1998·Molecular and Cellular Endocrinology·F ArcuriM Cintorino
Feb 27, 2017·Molecular and Cellular Endocrinology·Francesco DamianiJane E Norman
Aug 1, 2013·Physiological Reviews·Karen ChapmanJonathan Seckl
Apr 10, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Elif GuzelFrederick Schatz
Sep 15, 2005·Endocrine Reviews·Henry N JabbourHilary O D Critchley
Oct 17, 2002·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·T RosenC J Lockwood
Mar 18, 2006·Human Reproduction·Daniel M Campagne
Nov 22, 2001·The Journal of Biological Chemistry·Jennifer K RicherKathryn B Horwitz
Oct 6, 2009·Brain, Behavior, and Immunity·Khalil Karimi, Petra Clara Arck
Jan 14, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Anne E King, Hilary O D Critchley

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