PMID: 2508091Oct 1, 1989Paper

Expression of 12 rabbit IgA C alpha genes as chimeric rabbit-mouse IgA antibodies

Proceedings of the National Academy of Sciences of the United States of America
R D SchneidermanK L Knight

Abstract

Serologic analysis of rabbit secretory IgA initially identified two subclasses of IgA, IgA-f and IgA-g. Recent molecular genetic studies have resulted in the identification and cloning of 13 genes encoding the constant region (C) of rabbit IgA heavy chains. Each of these 13 C alpha genes, C alpha 1-C alpha 13, was subcloned into an expression vector containing the VDJ (V, variable; D, diversity; J, joining) gene of a dansyl (DNS)-binding hybridoma antibody. The alpha heavy-chain constructs were transfected into SP2/0 cells producing murine light chains with specificity for DNS. Of the 13 resulting transfectomas, 12 were shown by ELISA to secrete DNS-binding chimeric rabbit-mouse IgA molecules. By immunoblot analysis, the 12 IgA-producing transfectomas were shown to secrete alpha chains ranging in size from 60 to 72 kDa. These data suggest that rabbit IgA may be composed of as many as 12 IgA isotypes. This is in marked contrast to mouse and human, in which only 1 and 2 IgA isotypes, respectively, are found. Serologic analyses, using anti-IgA-f and anti-IgA-g alloantisera, revealed that 11 of the 12 transfectoma IgAs reacted with anti-IgA-f and not with anti-IgA-g antibodies and that one reacted with anti-IgA-g and not with anti-...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1985·Annual Review of Immunology·M E Koshland
Dec 1, 1987·Veterinary Immunology and Immunopathology·K L KnightR C Burnett
Dec 1, 1974·The Journal of Clinical Investigation·A G PlautJ D Capra
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H PotterP Leder
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R C Mulligan, P Berg

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Citations

Jan 1, 1992·Immunogenetics·T C Tobin-Janzen, J E Womack
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·K R Chintalacharuvu, S L Morrison
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M KingzetteK L Knight
Dec 17, 2014·Retrovirology·Mingkui Zhou, Ruth M Ruprecht
Aug 16, 2005·Developmental and Comparative Immunology·Rose G MageKatherine L Knight
Dec 6, 2017·Journal of Molecular Recognition : JMR·Izabela BehrendtSylwia Rodziewicz-Motowidło
Jan 1, 1997·International Reviews of Immunology·K L Knight, C R Winstead
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helga Spieker-PoletKatherine L Knight
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·V V VolginaK L Knight

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