PMID: 7525828Oct 1, 1994Paper

Expression of 17 beta-hydroxysteroid dehydrogenase in human granulosa cells: correlation with follicular size, cytochrome P450 aromatase activity and oestradiol production

The Journal of Endocrinology
S GhersevichR Vihko

Abstract

The aim of this study was to examine the expression and regulation of type 1 17 beta-hydroxysteroid dehydrogenase (type 1 17-HSD) enzyme protein and mRNA, and 17-HSD activity in human granulosa cells. The cells were obtained from patients taking part in an in vitro fertilization programme. The cells from each patient were divided into two groups: cells obtained from preovulatory follicles (LGC = granulosa cells from large follicles > or = 18 mm in diameter), and cells from other visible follicles (SGC = granulosa cells from small follicles, less than 15 mm in diameter). The identity of 17-HSD enzyme protein expressed in human granulosa cells with placental cytosolic 17-HSD (type 1 17-HSD) was assessed by immunoblot analysis using polyclonal antibodies, and the enzyme was immunolocalized in the cytoplasm of granulosa cells. Type 1 17-HSD protein concentration, 17-HSD and cytochrome P450 aromatase (P450arom) activities and oestradiol (OE2) production in cells from LGC were significantly lower than the corresponding values obtained in SGC in the same patient (paired t-test). The type 1 17-HSD protein concentration, 17-HSD activity and P450arom activity were 140 +/- 16% (mean +/- S.E.M.), 121 +/- 22% and 113 +/- 26% higher in cells...Continue Reading

Citations

Mar 1, 2012·Journal of Physiology and Biochemistry·Mirja RotinenIgnacio Encío
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M PoutanenR Vihko
Apr 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·P VihkoS Törn
Mar 18, 2004·Molecular and Cellular Endocrinology·P VihkoV Isomaa
Mar 31, 2004·Toxicology and Applied Pharmacology·R L PetersonA J Dorner
Feb 13, 2001·Molecular and Cellular Endocrinology·S GhersevichP Vihko
Sep 21, 2002·Molecular and Cellular Endocrinology·Tommi E VaskivuoJuha S Tapanainen
Nov 9, 2000·The Journal of Steroid Biochemistry and Molecular Biology·S GhersevichP Vihko
Dec 22, 1999·Breast Cancer Research and Treatment·M MiettinenP Vihko
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M W SawickiD Ghosh
Aug 31, 2011·Molecular and Cellular Endocrinology·Kaisa HuhtinenMatti Poutanen
Jan 25, 2005·The Journal of Steroid Biochemistry and Molecular Biology·P SoronenP Vihko
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Janne HakkarainenMatti Poutanen
May 29, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Janne HakkarainenMatti Poutanen
Mar 24, 2005·The Journal of Clinical Endocrinology and Metabolism·Tommi E VaskivuoJuha S Tapanainen
Jan 27, 2018·Endocrine-related Cancer·Päivi JärvensivuMatti Poutanen
Sep 15, 2005·Reproductive Toxicology·Laxmipriya P Nampoothiri, Sarita Gupta
Nov 26, 2008·Molecular and Cellular Endocrinology·Gabriele Moeller, Jerzy Adamski
Nov 26, 2008·Molecular and Cellular Endocrinology·Tarja LamminenMatti Poutanen

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